Evidence map›Paper›PMID 41895913›Full record

ArticleMethods in enzymology2026

Expression and purification of Steroidogenic Factor-1 (NR5A1) nuclear receptor ligand binding domain complexed with the signaling phosphoinositide lipid PI(3,4,5)P

Alexis N Campbell, Alexander J Cutright, Jessica Martin, Arwa Kheda, Mong Na Claire Loi, Raymond D Blind

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Alexis N CampbellDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, United States.
Alexander J CutrightDivision of Diabetes, Department of Medicine, Endocrinology & Metabolism, Vanderbilt University Medical Center, Nashville, TN, United States.
Jessica MartinDivision of Diabetes, Department of Medicine, Endocrinology & Metabolism, Vanderbilt University Medical Center, Nashville, TN, United States.
Arwa KhedaDivision of Diabetes, Department of Medicine, Endocrinology & Metabolism, Vanderbilt University Medical Center, Nashville, TN, United States.
Mong Na Claire LoiDivision of Diabetes, Department of Medicine, Endocrinology & Metabolism, Vanderbilt University Medical Center, Nashville, TN, United States.
Raymond D BlindDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, United States; Division of Diabetes, Department of Medicine, Endocrinology & Metabolism, Vanderbilt University Medical Center, Nashville, TN, United States. Electronic address: ray.blind@vanderbilt.edu.

Funding

Structure and function of phospholipids and inositol phosphates in the nucleus.R35GM156389 · NIGMS · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Raymond Daniel Blind · 2025 to 2026
$1.4M
Liver and kidney responses to bilirubin as a nuclear receptor hormoneR01DK141801 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Raymond Daniel Blind · 2025 to 2026
$761k
NIDDK NIH HHS R01 DK141801NIGMS NIH HHS R35 GM156389
6 · The paper itself

Abstract

Steroidogenic Factor-1 (SF-1, NR5A1) is an orphan nuclear receptor that plays a critical role in proper development and adult function of the adrenals, gonads and the ventral medial hypothalamus in the brain. Structural studies have revealed that the SF-1 ligand-binding domain is capable of interaction with glycerophospholipids, such as phosphoinositides, in its binding pocket. Of these, the phosphoinositide PI(3,4,5)P

Indexed as

Phosphatidylinositol PhosphatesSteroidogenic Factor 1AnimalsBinding SitesGene ExpressionHumansLigandsPhosphatidylinositolsProtein BindingProtein DomainsRecombinant ProteinsLigandsNR5A1 protein, humanPhosphatidylinositol PhosphatesPhosphatidylinositolsRecombinant ProteinsSteroidogenic Factor 1Nuclear phosphoinositides protein purification of nuclear receptor SF-1PhosphatidylinositolPI(3,4,5)P3PI(4,5)P2

Identifiers

PMID41895913
PMCPMC13096889

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.