Evidence mapPaperPMID 41898558Full record

ArticleInternational journal of molecular sciences2026

Integration of Network Pharmacology and Molecular Docking Together with an In Vitro Nitric Oxide Inhibition for the Insight for Antipyretic Effects of Benjalokawichian, the Thai Traditional Polyherbal Remedy.

Chinnaphat Chaloemram, Ruchilak Rattarom, Anake Kijjoa, Somsak Nualkaew

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chinnaphat ChaloemramDoctor of Philosophy in Pharmacy Program, Faculty of Pharmacy, Mahasarakham University, Kantharawichai 44150, Maha Sarakham, Thailand.ORCID 0000-0003-3941-4289
Ruchilak RattaromPharmaceutical Chemistry and Natural Product Research Unit, Faculty of Pharmacy, Mahasarakham University, Kantharawichai 44150, Maha Sarakham, Thailand.ORCID 0000-0002-3477-0121
Anake KijjoaSchool of Medicine and Biomedical Sciences Abel Salazar (ICBAS) and CIIMAR, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.ORCID 0000-0002-3321-1061
Somsak NualkaewPharmaceutical Chemistry and Natural Product Research Unit, Faculty of Pharmacy, Mahasarakham University, Kantharawichai 44150, Maha Sarakham, Thailand.ORCID 0000-0002-6168-0260

Funding

Mahasarakham University 025/2569
6 · The paper itself

Abstract

Benjalokawichian (BLW) is a classic antipyretic polyherbal remedy used in Thai traditional medicine (TTM) to reduce toxic fever (TF). This study aimed to shed light on the mechanisms of action and identify bioactive components of BLW responsible for TF treatment. The methods that combine network pharmacology, molecular docking, and the inhibition of nitric oxide (NO) production in LPS-induced RAW 264.7 were employed for these objectives. Network pharmacology served as a means to identify 15 potential bioactive compounds, 88 possible therapeutic targets, and 4 hub genes related to BLW. Among the significant targets, TNF, PTGS2, STAT3, and NFKB1 were closely linked to the metabolic pathways of phenylalanine, arachidonic acid, and tyrosine, which are vital in managing infections, inflammation, proliferation, and apoptosis in the TF microenvironment. Additionally, molecular docking analysis indicated that core compounds displayed strong binding affinities for the key targets, with binding energies ranging between -4.5 and -11.1 kcal/mol. The in vitro assay demonstrated that BLW extract significantly inhibited NO production in LPS-activated RAW 264.7 macrophages, presenting an IC

Indexed as

AntipyreticsMedicine, Thai TraditionalMolecular Docking SimulationNetwork PharmacologyNitric OxidePlant ExtractsAnimalsLipopolysaccharidesMacrophagesMiceRAW 264.7 CellsAntipyreticsLipopolysaccharidesNitric OxidePlant Extractsanti-inflammatoryBenjalokawichianHa-Rakmolecular dockingnetwork pharmacologyNO inhibitiontoxic fever

Identifiers

PMID41898558
PMCPMC13026877

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.