Evidence mapPaperPMID 41898652Full record

ArticleInternational journal of molecular sciences2026

Targeting Activated Pathways in Doxorubicin-Resistant TNBC Alters Signaling, Survival and EMT: A Double-Edged Sword.

Irem Dogan Turacli, Sahika Cingir Koker, Kubra Paspal Eroglu, Banu Yalcin

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Irem Dogan TuracliFaculty of Medicine, Department of Medical Biology, Ufuk University, Ankara 06520, Turkey.ORCID 0000-0002-3791-3538
Sahika Cingir KokerFaculty of Medicine, Department of Medical Biology, Ufuk University, Ankara 06520, Turkey.ORCID 0000-0002-8529-4532
Kubra Paspal ErogluFaculty of Medicine, Department of Medical Biology, Ufuk University, Ankara 06520, Turkey.
Banu YalcinOgretmen Naime Tomek Research Laboratory (ONTAL), Faculty of Medicine, Ufuk University, Ankara 06520, Turkey.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Triple-negative breast cancer (TNBC) poses significant therapeutic challenges due to the limited availability of targeted treatment options and the development of resistance to chemotherapy, including doxorubicin (DOX). The objective of this study was to investigate the impact of inhibiting activated pathways in DOX-resistant TNBC and examine the effects on MAPK and PI3K/Akt signaling pathways, cell cycle regulation, and the regulators of the epithelial-mesenchymal transition (EMT) process. Continuous exposure of cells to increasing concentrations of DOX resulted in the selection of resistant cells that exhibited EMT characteristics. We assessed the expression levels of markers related to cell death, survival, mitophagy pathways and EMT using Western blotting and qPCR in both sensitive and resistant cells with activated-pathway inhibitor treatments. Additionally, we demonstrated differences in migration capacity between resistant and sensitive cells with or without inhibitor treatments. It was found that MEK inhibition was less effective than PI3K inhibition in both sensitive and resistant cells. Expression analyses clearly demonstrated that resistant cells exhibited more aggressive behavior, as indicated by EMT- and survival-related gene expressions. The combination of MEK and PI3K inhibitors was more effective in shutting down these signals in both cell types. The ability to induce EMT in DOX-resistant cells revealed that one form of resistance might combine with another, acting as a mediator for cellular switch. Although drug resistance and various inhibitors reduce the proliferative capacity of cells and related parameters, resistance contributes to the acquisition of metastatic characteristics.

Indexed as

DoxorubicinDrug Resistance, NeoplasmEpithelial-Mesenchymal TransitionSignal TransductionTriple Negative Breast NeoplasmsAntibiotics, AntineoplasticCell Line, TumorCell MovementCell SurvivalFemaleHumansPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktAntibiotics, AntineoplasticDoxorubicinPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktdoxorubicinEMTMEKPI3KTNBC

Identifiers

PMID41898652
PMCPMC13026208

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.