Kombucha SCOBY as a Fermentation-Derived Biofilm Matrix: Species-Resolved Microbial Communities and Multidimensional In Vitro Bioactivities.

Anita Hartono, Kyra Singgih Palupi, Riza-Arief Putranto, Antonello Santini, Fahrul Nurkolis

Abstract read

In one paragraph

Article in Polymers, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Anita HartonoMambucha Scobylogy International, Jakarta 14240, Indonesia.

Kyra Singgih PalupiBiology Department, Faculty of Science and Technology, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), Yogyakarta 55281, Indonesia.ORCID 0009-0001-1367-785X

Riza-Arief PutrantoDepartment of Biotechnology, Faculty of Medical Sciences, Esa Unggul University, Jakarta 11510, Indonesia.ORCID 0000-0002-1327-1200

Antonello SantiniDepartment of Pharmacy, University of Napoli Federico II, Via Domenico Montesano, 49, 80131 Napoli, Italy.ORCID 0000-0001-5505-3327

Fahrul NurkolisInstitute for Research and Community Service, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), Yogyakarta 55281, Indonesia.ORCID 0000-0003-2151-0854

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Kombucha fermentation is driven by a Symbiotic Culture of Bacteria and Yeast (SCOBY), a cellulose-rich biofilm that hosts a complex microbial consortium. While most kombucha studies focus on the liquid beverage, the SCOBY pellicle itself remains underexplored, particularly with respect to species-level microbial resolution and its intrinsic biological activities. In this study, a commercial kombucha SCOBY was characterized using full-length 16S rRNA gene and ITS amplicon sequencing based on Oxford Nanopore Technology, enabling species-level taxonomic resolution. In parallel, hydroalcoholic and aqueous extracts of dried SCOBY biomass were evaluated for in vitro antioxidant activity (DPPH and ABTS assays), antidiabetic-related enzyme inhibition (α-glucosidase and dipeptidyl peptidase-4, DPP4), and anti-aging-related enzyme inhibition (tyrosinase and elastase). The SCOBY bacterial community was strongly dominated by acetic acid bacteria, with

Indexed as

anti-aging enzymesantioxidant activityfull-length 16S–ITS sequencingkombucha SCOBYOxford Nanopore Technologyα-glucosidase and DPP4 inhibition

Identifiers

PMID41901895

DOI10.3390/polym18060764

PMCPMC13030292

What Socratic holds

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