ArticleMicrobial biotechnology2026
Engineering Yeast Extracellular Vesicle Biogenesis Through Rewiring Membrane Trafficking Pathways.
Article in Microbial biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
12 authors.
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Abstract
Extracellular vesicles (EVs) are emerging as versatile therapeutic platforms, yet the mechanisms governing their biogenesis in yeast remain incompletely understood. Saccharomyces cerevisiae, a well-characterised and safe microbial chassis, naturally secretes abundant EVs and provides an attractive system for mechanistic dissection and engineering. Here, we establish S. cerevisiae as a tractable model for elucidating EV cargo loading. By combining multicopy expression of chicken interferon-λ (ChiIFN-λ) with cell wall perturbation, we achieved a tenfold increase in EV yield and efficient incorporation of ChiIFN-λ into EVs. Quantitative proteomics identified 1555 EV-associated proteins, including 501 predicted transmembrane proteins derived from multiple organelles. ChiIFN-λ overexpression and cell wall stress selectively reduced the abundance of key vesicle trafficking regulators, including SNARE, ESCRT and Rab proteins, indicating reprogramming of intracellular membrane trafficking pathways. Functional analyses further demonstrated that the SNARE proteins Sso2 and Nyv1 are enriched in the EV membrane and modulate EV size distribution and subpopulation composition. Together, these results reveal conserved protein-sorting machinery underlying yeast-derived extracellular vesicles (YDEVs) biogenesis and establish S. cerevisiae as a powerful platform for engineered EV production.
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