Evidence map›Paper›PMID 41907416›Full record

ArticleiScience2026

Perfusion microbioreactor for CAR-Treg manufacturing.

William Edwards, Ningjia Sun, Yikai Wang, Yuhan Lu, Cong Wang, Daniela Mastronicola, Cristiano Scottà, Marco Romano, Cesare M Cejas, Antoine Espinet and 2 more

Abstract read
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Microbioreactor design for eukaryotic cell-based biomedical applications.Frontiers in bioengineering and biotechnology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

William EdwardsCentre for Craniofacial and Regenerative Biology, King's College London, London SE1 9RT, UK.
Ningjia SunCentre for Craniofacial and Regenerative Biology, King's College London, London SE1 9RT, UK.
Yikai WangCentre for Craniofacial and Regenerative Biology, King's College London, London SE1 9RT, UK.
Yuhan LuCentre for Craniofacial and Regenerative Biology, King's College London, London SE1 9RT, UK.
Cong WangCentre for Craniofacial and Regenerative Biology, King's College London, London SE1 9RT, UK.
Daniela MastronicolaPeter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, Faculty of Life Sciences & Medicine, King's College London, London SE1 7EH, UK.
Cristiano ScottàPeter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, Faculty of Life Sciences & Medicine, King's College London, London SE1 7EH, UK.
Marco RomanoPeter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, Faculty of Life Sciences & Medicine, King's College London, London SE1 7EH, UK.
Cesare M CejasMFX (MicrofluidX) Ltd., Stevenage Bioscience Catalyst, Gunnels Wood Road, Stevenage SG1 2FX, UK.
Antoine EspinetMFX (MicrofluidX) Ltd., Stevenage Bioscience Catalyst, Gunnels Wood Road, Stevenage SG1 2FX, UK.
Giovanna LombardiPeter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, Faculty of Life Sciences & Medicine, King's College London, London SE1 7EH, UK.
Ciro ChiappiniCentre for Craniofacial and Regenerative Biology, King's College London, London SE1 9RT, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Manufacturing cell and gene therapies (CGTs) at scale presents challenges in cost, product consistency, and adaptability to personalized treatments. Traditional large-volume bioreactors are designed to support cell growth through controlled nutrient delivery and gas exchange, but are poorly suited to the decentralized, small-batch production required for personalized therapies such as chimeric antigen receptor (CAR) T cells. To address this, we have developed the KCL-Microbioreactor (K-MBR), a closed microbioreactor platform based on microfluidic principles. Engineered in polydimethylsiloxane (PDMS), the K-MBR combines spatial confinement, semi-continuous perfusion, and integrated viral transduction in a compact footprint, enabling efficient gene delivery and robust expansion of therapeutic cells. We demonstrate the platform's utility by generating functional CAR-Tregs targeting HLA-A2, achieving a 92% increase in yield compared to conventional methods. The K-MBR offers a streamlined solution for CGT manufacturing, with potential to reduce production costs and enhance scalability across a broad range of cell therapies.

Indexed as

Biological sciences

Identifiers

PMID41907416
PMCPMC13019498

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.