Evidence map›Paper›PMID 41907427›Full record

ArticleiScience2026

Single-cell transcriptomic analyses reveal angiogenic vascular responses to chronic cerebral hypoperfusion.

Jiajing Shan, Ruyu Shi, Liyuan Jiang, Connor Dufort, Wanying Miao, Ting-Wei Mai, Junxuan Lyu, Julieta Barreiro, Kong Chen, Rehana K Leak and 2 more

Abstract read
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jiajing ShanDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Ruyu ShiDepartment of Human Genetics, School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA.
Liyuan JiangDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Connor DufortDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Wanying MiaoDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Ting-Wei MaiDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Junxuan LyuDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Julieta BarreiroDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Kong ChenDepartment of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Rehana K LeakGraduate School of Pharmaceutical Sciences, Duquesne University, Pittsburgh, PA, USA.
Jun ChenDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Xiaoming HuDepartment of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.

Funding

Adiponectin on cerebrovascular regulation in vascular cognitive impairment and dementia (VCID)R01NS124673 · NINDS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Jun Chen, Xiaoming Hu · 2022 to 2026
$1.9M
White Matter Injury and Repair in Vascular Cognitive Impairment and DementiaRF1NS131169 · NINDS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HU, XIAOMING · 2023 to 2023
$1.4M
BLRD VA I01 BX005290NINDS NIH HHS R01 NS124673NINDS NIH HHS RF1 NS131169
6 · The paper itself

Abstract

Elucidating the mechanisms underlying vascular injury and repair may guide development of therapies against vascular cognitive impairment and dementia (VD). We employed single-cell RNA sequencing to map cell populations and explore vascular responses in mouse brains collected 42 days after asymmetric common carotid artery stenosis (ACAS)-induced chronic cerebral hypoperfusion. A unique tip cell type was identified among endothelial cell (EC) clusters and validated by immunostaining. Gene Ontology analyses suggested tip cell enrichment in angiogenesis and the involvement of Apln/Aplnr signaling. Immunoblotting confirmed an increase in apelin (Apln) protein after ACAS. In EC cultures, [Pyr1]-Apelin-13 (Apln13), a selective endogenous apelin receptor agonist, enhanced EC proliferation, migration, and tube formation. Treatment with Apln13 also improved angiogenesis, white matter integrity, and cognitive functions in ACAS mice. Cell-cell interaction analyses highlighted astrocyte-tip cell crosstalk via Vegfa-Vegfr interactions.

Indexed as

Integrative aspects of cell biologyTranscriptomicsVascular remodeling

Identifiers

PMID41907427
PMCPMC13019947

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.