Evidence map›Paper›PMID 41916059›Full record

ArticlePoultry science2026

Integrated analysis of transcriptome and metabolomic uncover molecular mechanisms of ovarian aging in laying hens.

Huiting He, Qing Ma, Xingyu Zhang, Chunjie Zhou, Fangtan Liu, Yinglin Lu, Minli Yu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Huiting HeDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Xuanwu District, Nanjing, Jiangsu Province 210095, PR China.
Qing MaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Xuanwu District, Nanjing, Jiangsu Province 210095, PR China.
Xingyu ZhangDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Xuanwu District, Nanjing, Jiangsu Province 210095, PR China.
Chunjie ZhouDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Xuanwu District, Nanjing, Jiangsu Province 210095, PR China.
Fangtan LiuDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Xuanwu District, Nanjing, Jiangsu Province 210095, PR China.
Yinglin LuDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Xuanwu District, Nanjing, Jiangsu Province 210095, PR China.
Minli YuDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Xuanwu District, Nanjing, Jiangsu Province 210095, PR China. Electronic address: yuminli@njau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ovarian aging shortens the productive lifespan and diminishes economic value of poultry, yet its mechanisms are unclear. This study investigated ovarian aging using transcriptomics and metabolomics. Result suggested that the number of primitive, primary, and secondary follicles significantly reduced in aged hens, while atretic follicles increased. Ovaries showed fibrosis with increased collagen deposition, and the thickness of follicle granulosa cell layers was remarkably reduced, exhibiting a disorganized and loose structure. The mitochondria of granulosa cells in aged hens exhibited vacuolation and sparse cristae. Additionally, antioxidant (GSH-Px, SOD) and reproductive hormone (AMH, E2) levels were significantly lower in aged hens (p < 0.05). Transcriptomic analysis revealed altered pathways including PPAR, ECM-receptor interaction, and cytokine-cytokine receptor interaction in aging ovaries. Metabolomic profiling further implicated biosynthesis of unsaturated fatty acids and purine metabolism pathways in aging ovaries. Integrated analysis revealed that FABP4 was a critical mediator linking lipid metabolism and inflammation, and it showed a negative correlation with conjugated linoleic acids (CLA) and cis-4,7,10,13,16,19-docosahexaenoic acid (DHA), suggesting therapeutic potential of their dietary supplementation. To understand how lipid metabolism dysregulation induced ovarian aging, the expression of key genes was assessed by qRT-PCR. The expression of senescence markers (p16, p21, p53), apoptosis-related genes (Bax/Bcl-2) (p < 0.001), autophagy-related gene (p62) (p < 0.01) and inflammation-related genes (IL6, TNFα, NOS2) (p < 0.01) in aged hens signifficantly increased, while the expression of autophagy-related genes (LC3B/LC3A) decreased (p < 0.05). This work provides a theoretical basis for strategies to delay ovarian aging and improve reproductive efficiency.

Indexed as

AgingChickensMetabolomeOvaryTranscriptomeAnimalsFemaleGene Expression ProfilingMetabolomicsAgingInflammationNon-targeted metabolomicOvaryTranscriptome

Identifiers

PMID41916059
PMCPMC13068545

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.