Evidence map›Paper›PMID 41920744›Full record

ArticleSTAR protocols2026

Protocol for culture, drug treatment, and dissociation of patient-derived 3D ovarian cancer microspheroids for flow cytometry.

Mariam Haffa, Karolina Juhani, Greta Gudoityte, Rita Hutyra-Gram Ötvös, Emelie Wallin, Olli-Pekka Kallioniemi, Josefin Fernebro, Ulrika Joneborg, Brinton Seashore-Ludlow

Abstract read
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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mariam HaffaScience for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden. Electronic address: mariam.haffa@ki.se.
Karolina JuhaniScience for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden.
Greta GudoityteScience for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden.
Rita Hutyra-Gram ÖtvösScience for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden.
Emelie WallinDepartment of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden; Department of Pelvic Cancer, Theme Cancer, Karolinska University Hospital, 17176 Stockholm, Sweden.
Olli-Pekka KallioniemiScience for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden; Institute for Molecular Medicine Finland, University of Helsinki, 00290 Helsinki, Finland.
Josefin FernebroDepartment of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden; Department of Women's and Children's Health, Division of Obstetrics and Gynecology, Karolinska Institutet, 17177 Solna, Sweden.
Ulrika JoneborgDepartment of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden; Department of Women's and Children's Health, Division of Obstetrics and Gynecology, Karolinska Institutet, 17177 Solna, Sweden.
Brinton Seashore-LudlowScience for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, 17177 Solna, Sweden. Electronic address: brinton.seashore-ludlow@ki.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell technologies provide insights into tumor heterogeneity and treatment response. Here, we present a protocol to culture patient-derived ovarian tumor cells as 3D microspheroids. We describe steps for preparing multi-well dishes and isolating ovarian tumor cells for spheroid generation and culture. We then detail procedures for drug treatment, recovery, and dissociation of spheroids followed by single-cell analysis via flow cytometry. This scalable and cost-efficient protocol largely preserves the cellular composition of the original material.

Indexed as

Cell Culture TechniquesFlow CytometryOvarian NeoplasmsSpheroids, CellularAntineoplastic AgentsFemaleHumansSingle-Cell AnalysisTumor Cells, CulturedAntineoplastic AgentsCancerCell-based AssaysCell BiologyCell cultureCell isolationFlow CytometrySingle Cell

Identifiers

PMID41920744
PMCPMC13068562

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.