Evidence map›Paper›PMID 41922521›Full record

ArticleScientific reports2026

scRNA-seq reveals different cell clusters in the testes of Mongolian cattle and EGR1/FOS/JUN regulation in Sertoli cells.

Shuaipeng Gao, Shuyin Zhang, Hongrui Ren, Haoyu Yang, Li Wang, Jinxin He, Junzhen Zhang, GuoLi Zhang, Bayier Mengke, Lixian Zhu and 4 more

Abstract read
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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Shuaipeng GaoCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China.
Shuyin ZhangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China.
Hongrui RenCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China.
Haoyu YangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China.
Li WangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China.
Jinxin HeCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China.
Junzhen ZhangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China.
GuoLi ZhangCollege of Agriculture and Biology, Liaocheng University, Liaocheng, 252000, China.
Bayier MengkeAnimal Husbandry and Veterinary Technology Extension Center of Alxa in Inner Mongolia, Alxa, 750300, China.
Lixian ZhuAnimal Husbandry and Veterinary Technology Extension Center of Alxa in Inner Mongolia, Alxa, 750300, China.
Qige QiAnimal Husbandry and Veterinary Technology Extension Center of Alxa in Inner Mongolia, Alxa, 750300, China.
Sarengaowa AierqingAnimal Husbandry and Veterinary Technology Extension Center of Alxa in Inner Mongolia, Alxa, 750300, China.
Ruiwen FanCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China. ruiwenfan@163.com.
Muren HerridGrassland & Cattle Investment Co., Ltd., Hohhot, 011500, China. mherrid@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mongolian cattle maintain stable reproductive performance under long-term cold and nutrient-limited conditions, but the molecular basis of their testicular function remains unclear. In this research, a single-cell RNA-sequencing (scRNA-seq) atlas of Mongolian cattle testes was generated and analyzed. The results showed that scRNA-seq identified major germ and somatic cell types, in particular Sertoli cells, which was composed of four subclusters representation different developmental stages. Transcriptomic analysis and pseudotime reconstruction indicated that Early Growth Response 1 (EGR1) might be a potential regulator of Sertoli cell maturation. To investigate the function of EGR1 in Sertoli cells, the methods of siRNA knockdown, overexpression, immunohistochemistry, and ChIP–qPCR were used and the results showed that EGR1 could directly bind to and activate FOS and JUN, joining an EGR1–FOS/JUN-Nectin-2 regulatory pathway in Sertoli cells to maintain the reproductive capacity. The results provided insights into the cellular and molecular mechanisms underlying the reproductive performance of Mongolian cattle.

Indexed as

Early Growth Response Protein 1Proto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junSertoli CellsTestisAnimalsCattleGene Expression RegulationMaleRNA-SeqSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisEarly Growth Response Protein 1Proto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junEGR1Mongolian cattlescRNA-seqSertoli cells (SC)Testis

Identifiers

PMID41922521
PMCPMC13184098

What Socratic holds

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.