Evidence mapPaperPMID 41923172Full record

ArticleCancer & metabolism2026

Leveraging untargeted metabolomics in combination with machine learning to uncover novel insights into bladder cancer.

Abu Hena Mostafa Kamal, Vasanta Putluri, Tanmay Gandhi, Chandra Shekar R Ambati, Chandra Sekhar Amara, Karthik Reddy Kami Reddy, Meredith Lauren Spradlin, Amrit Koirala, Sachin B Jorvekar, Sandra L Grimm and 12 more

Abstract read
In one paragraph

Article in Cancer & metabolism, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Abu Hena Mostafa Kamal *Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Vasanta Putluri *Advanced Technology Cores, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
Tanmay Gandhi *Advanced Technology Cores, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
Chandra Shekar R AmbatiAdvanced Technology Cores, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
Chandra Sekhar AmaraDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Karthik Reddy Kami ReddyDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Meredith Lauren SpradlinDepartment of Surgery, Baylor College of Medicine, Houston, TX, USA.
Amrit KoiralaDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Sachin B JorvekarDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Sandra L GrimmDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Dexue FuDivision of Urology, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.
Krishna ParsawarAnalytical and Biological Mass Spectrometry Core, University of Arizona, Tucson, AZ, USA.
Felice de JongIROA Technologies, Chapel Hill, NC, USA.
Chris BeecherIROA Technologies, Chapel Hill, NC, USA.
Subrata SenDepartment of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Seth P LernerScott Department of Urology, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.
M Minhaj SiddiquiDivision of Urology, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.
Yair LotanDepartment of Urology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Livia S EberlinDepartment of Surgery, Baylor College of Medicine, Houston, TX, USA.
Arun SreekumarDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
Cristian CoarfaDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA. coarfa@bcm.edu.
Nagireddy PutluriDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA. putluri@bcm.edu.

Funding

Tumor Model and Biospecimen Repository CoreU54CA274321 · UNIVERSITY OF TX MD ANDERSON CAN CTR · 2025 to 2025
$1.2M
Decoding tumor metabolic and immunologic interactions driving different biological subtypes in patients with bladder cancer.R01CA282282 · BAYLOR COLLEGE OF MEDICINE · 2025 to 2025
$615k
NCI NIH HHS R01 CA282282NCI NIH HHS U54 CA274321
6 · The paper itself

Abstract

backgroundUntargeted metabolomics has emerged as a powerful approach to uncover metabolic dysregulation associated with cancer progression. When integrated with a machine learning strategy it facilitates the discovery of key metabolic pathways and predictive biomarkers with high diagnostic and prognostic value.

methodsIn this study, we employed liquid chromatography coupled to high-resolution Tribrid Orbitrap mass spectrometry to perform comprehensive metabolic profiling of bladder cancer (BLCA) as well as predict invasiveness of the disease.

resultsBy leveraging both in-house retention time-based MS/MS spectral libraries and commercial databases, we robustly identify over 2000 metabolites. In addition, this platform allows identification of novel pathways highlighting metabolic vulnerabilities in BLCA. The application of machine learning algorithms and advanced computational modeling uncovered metabolic signatures that differentiate BLCA from adjacent normal/benign samples and distinguish muscle-invasive from non-muscle-invasive bladder cancer. Our integrative analytical pipeline addresses key challenges in metabolomics-including high dimensionality, metabolite annotation, and biological variability-through feature selection and predictive modeling. We identify candidate metabolic markers with strong potential for early detection and characterize invasiveness of the disease and identify potential therapeutic target pathways.

conclusionsThis work highlights the power of combining untargeted metabolomics with machine learning to map the metabolic landscape of BLCA and to accelerate the development of precision diagnostics and future therapeutic strategies.

Indexed as

Bladder cancerMachine learningOrbitrap IQ-XUntargeted metabolomics

Identifiers

PMID41923172
PMCPMC13040883

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.