Evidence map›Paper›PMID 41923299›Full record

ArticleChemistryOpen2026

Extraction and Optimization of Fucoidan From Sargassum muticum Using Acidic Eutectic Solvents and Evaluation of Their Effects on Fibroblast Cell Lines.

Marina Leite, Francisca Silva, Antônio Lima, Afonso Cunha, Thiago Cahú, Helga Gomes, Paulo A S Mourão, Mauro S G Pavão, Bernardo D Ribeiro

Abstract read
In one paragraph

Article in ChemistryOpen, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marina LeiteDepartment of Biochemical Enginnering School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0009-0007-6060-8324
Francisca SilvaInstitution of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0002-0928-4690
Antônio LimaInstitution of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0003-0106-923X
Afonso CunhaInstitution of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0009-0005-9063-2558
Thiago CahúInstitution of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0002-7853-0613
Helga GomesInstitution of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0002-4373-6152
Paulo A S MourãoInstitution of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0001-6254-4124
Mauro S G PavãoInstitution of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0002-1336-4271
Bernardo D RibeiroDepartment of Biochemical Enginnering School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID 0000-0001-8621-156X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fucoidan, a sulfated polysaccharide extracted from the cell wall of macroalgae such as S. muticum, exhibits promising biological properties. This study investigated a green extraction using deep eutectic solvents (DESs), with an emphasis on process optimization and bioactive evaluation of fucoidan. The most efficient DES was lactic acid with glycerol (LacAc:Gly) at 60°C, with 4% water and a solid/liquid ratio of 0.1167, resulting in a yield of 6.6%, a sulfate content of 0.170 mg/mg, and a uronic acid content of 0.023 mg/mg. The chemical and structural composition of fucoidan were evaluated by colorimetric methods, FTIR, and NMR. Antioxidant activity was assessed by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate (ABTS, 25.84%), 2-diphenyl-1-pricylhydrazyl (DPPH, 30.45%), and ferric reducing antioxidant power (FRAP, 690 µg/µg) assays. Cellular assays with L929 fibroblasts showed that concentrations of 50 and 75 µg/mL stimulated cell viability, with 179% colony formation and 449 invasive cells, suggesting positive effects on cell proliferation and migration. Therefore, the results indicate that fucoidan extracted with LacAc:Gly has high bioactive potential, demonstrating the viability of green eutectic solvents as a sustainable alternative for the utilization of bioactive compounds from brown algae.

Indexed as

AntioxidantsDeep Eutectic SolventsFibroblastsPolysaccharidesSargassumAnimalsCell LineCell ProliferationCell SurvivalMiceAntioxidantsDeep Eutectic SolventsfucoidanPolysaccharidescellular regenerationdeep eutectic solventsfucoidanhealingSargassum muticum

Identifiers

PMID41923299
PMCPMC13045398

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.