Evidence map›Paper›PMID 41927052›Full record

ArticleClinical and experimental pediatrics2026

Thyroid peroxidase gene variants and susceptibility to congenital hypothyroidism and autoimmune thyroid disease among Egyptian pediatric cohort.

Hala M Sakhr, Mohammed H Hassan, Esraa Abbass Abdallah, Amira Mahmoud Ewis, Mohamed Hesham Mohamed, Shymaa Gaber Rizk

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Article in Clinical and experimental pediatrics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Hala M SakhrDepartment of Pediatrics, Faculty of Medicine, Qena University, Qena, Egypt. hala.sakhr@med.svu.edu.eg.
Mohammed H HassanDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Qena University, Qena, Egypt. mohammedhosnyhassaan@yahoo.com.
Esraa Abbass AbdallahDepartment of Clinical Pathology, Faculty of Medicine, Qena University, Qena, Egypt.
Amira Mahmoud EwisDepartment of Pediatrics, Faculty of Medicine, Qena University, Qena, Egypt.
Mohamed Hesham MohamedDepartment of Pediatrics, Faculty of Medicine, Qena University, Qena, Egypt.
Shymaa Gaber RizkDepartment of Pediatrics, Faculty of Medicine, Qena University, Qena, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Pediatric thyroid disorders arise from complex interactions among genetic susceptibility, immune dysregulation, and metabolic factors that influence their onset and severity. Purpose: This study aimed to evaluate the clinical, biochemical, micronutrient, and genetic determinants of thyroid dysfunction in children with particular emphasis on the thyroid peroxidase (TPO) Arg386His and Thr725Pro polymorphisms. Methods: This case-control study enrolled 150 Egyptian children aged 2-15 years, including 50 with congenital hypothyroidism (CH), 50 with autoimmune thyroiditis (AIT), and 50 controls. The participants underwent clinical assessments, routine laboratory investigations, thyroid function tests, and tests for selenium, copper, and thyroid autoantibodies (Tg Abs and TPO Abs). Following genomic DNA extraction from peripheral blood, the TPO gene polymorphisms (Arg386His and Thr725Pro) were analyzed using polymerase chain reaction-restriction fragment length polymorphism. Results: Children with thyroid disorders exhibit a higher prevalence of anemia and significantly lower ferritin levels. Profound micronutrient disturbances were evident and characterized by severe selenium deficiency and elevated copper concentrations. The genetic analysis demonstrated a strong association between the TPO exon-8 Arg386His polymorphism and pediatric thyroid disorders. The His allele and His-containing genotypes were significantly more frequent among cases, particularly in children with CH. A genotype-phenotype correlation revealed that the histidine/histidine (His/His) genotype was consistently associated with the highest serumthyroid-stimulating hormone levels in both CH and AIT, suggesting greater functional impairment of thyroid hormone synthesis. Distinct genetic patterns were observed between disease subtypes, with a predominance of the His allele in CH and the Arg allele in AIT. No variation was detected in the Thr725Pro polymorphism, and all participants exhibited the Thr/Thr genotype. Conclusion: These findings support a multifactorial model of pediatric thyroid disease, with the TPO Arg386His variant, particularly the His/His genotype, emerging as a key genetic contributor to disease susceptibility and severity.

Indexed as

Autoimmune thyroiditisCongenital hypothyroidismTPO gene polymorphisms

Identifiers

PMID41927052
PMCPMC13244060

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