Evidence map›Paper›PMID 41928786›Full record

ArticleResearch square2026

Epigenetic control of PDX1 and NGN3 by a computationally designed PRC2 inhibitor enforces pancreatic endocrine differentiation from pluripotent stem cells.

Laura Crisa, Shiri Levy, Dan Mar, Karol Bomsztyk, Stephanie Battle, Sina Gharib, Cecilia Lopez-Martinez, Patrick McGrath, Julie Mathieu, Marie Regier and 2 more

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In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Laura CrisaDepartment of Medicine, University of Washington, Seattle, WA, USA.
Shiri LevyDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Dan MarDepartment of Medicine, University of Washington, Seattle, WA, USA.
Karol BomsztykDepartment of Medicine, University of Washington, Seattle, WA, USA.
Stephanie BattleDepartment of Natural Sciences, Bowie State University, Bowie, MD, USA.
Sina GharibDepartment of Medicine and Computational Medicine Core, University of Washington, Seattle, WA, USA.ORCID 0000-0002-2480-4367
Cecilia Lopez-MartinezDepartment of Medicine and Computational Medicine Core, University of Washington, Seattle, WA, USA.
Patrick McGrathCharles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, USA.ORCID 0000-0002-1498-371X
Julie MathieuDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Marie RegierDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Hannele Ruohola-BakerDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0002-5588-4531
Vincenzo CirulliDepartment of Medicine, University of Washington, Seattle, WA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Directed differentiation of pluripotent stem cells (PSCs) into pancreatic islets is a cornerstone strategy for diabetes cell therapy. This process relies on growth factor-driven activation of core transcriptional regulators, notably PDX1 and NGN3, to restrict the multi-lineage potential of definitive endoderm to pancreatic progenitors and endocrine cell types. Yet differentiation efficiency and lineage fidelity vary markedly across PSC lines. Here, we demonstrate that a dominant constraint is persistent Polycomb Repressive Complex 2 (PRC2)-mediated epigenetic repression at the

Identifiers

PMID41928786
PMCPMC13042171

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.