Evidence map›Paper›PMID 41933083›Full record

ArticleJournal of artificial organs : the official journal of the Japanese Society for Artificial Organs2026

Cytochrome P450-derived metabolites of docosahexaenoic acid or arachidonic acid enhance contractility of cultured rat cardiomyocytes: a pilot study.

Hikari Kawakami, Daisuke Sato, Masaki Sazuka, Saki Tsuchida, Atsuyoshi Nishina, Takao Nakamura, Masataka Kusunoki, Kazuhiko Tsutsumi, Zhonggang Feng

Abstract read
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In one paragraph

Article in Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hikari Kawakami *Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, 992-8510, Japan.
Daisuke Sato *Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, 992-8510, Japan. d_sato@yz.yamagata-u.ac.jp.ORCID http://orcid.org/0000-0002-6923-832X
Masaki SazukaFaculty of Health and Nutrition, Yamagata Prefectural Yonezawa University of Nutrition Sciences, Yonezawa, Japan.
Saki TsuchidaDepartment of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, 992-8510, Japan.
Atsuyoshi NishinaDepartment of Health and Nutrition, Tokai Gakuen University, Nagoya, Japan.
Takao NakamuraYamagata University, Yamagata, Japan.
Masataka KusunokiApollon Nagoya Exercise Therapy Center for Diabetes, Akishima Clinic, Nagoya, Japan.
Kazuhiko TsutsumiApollon Nagoya Exercise Therapy Center for Diabetes, Akishima Clinic, Nagoya, Japan.
Zhonggang FengDepartment of Mechanical Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Japan.

Funding

Takahashi Industrial and Economic Research Foundation 12-003-111Takahashi Industrial and Economic Research Foundation 13-003-027
6 · The paper itself

Abstract

Regenerative medicine is perceived as a new treatment for severe heart disease. However, cell-based regenerative therapy for cardiac tissue primarily contributes through its paracrine effect, with limited direct impact on contractile force. We have previously found that docosahexaenoic (DHA) and arachidonic (AA) acid could improve the contractility of cultured cardiomyocytes. On the other hand, the beneficial effect of their metabolites produced by cytochrome P450 (CYP) has not been clarified yet because CYPs are abundantly expressed in the liver. In the present study, we evaluated the effects of CYP-derived metabolite of DHA or AA on contractility of cultured rat cardiomyocytes. The cardiomyocytes were cultured in the medium which were supplemented each metabolite for 4 days, and then we evaluated contractile fraction, beat rate, and contraction velocity under a microscope. In addition, we fabricated collagen gel-based myocardial tissue, cultured them for 24 h in the metabolites-containing medium, and measured the contractile force generated by the tissue. 19,20-epoxydocosapentaenoic acid (EpDPE) enhanced both contractile fraction and contraction velocity of cardiomyocytes, whereas 22-hydroxydocosahexaenoic and 8,9-eicosatrienoic acids increased contractile fraction only, and 18- and 20-hydroxyeicosatetraenoic acids increased contraction velocity. Furthermore, 19,20-EpDPE tended to increase the contractile force of myocardial tissue, whereas none of the other metabolites produced a comparable effect. These results indicate that some DHA and AA metabolites can improve contractile fraction as well as contraction velocity of cardiomyocytes in culture; however, an appreciable contribution to contractile force within 24 h is not anticipated.

Indexed as

Arachidonic AcidCytochrome P-450 Enzyme SystemDocosahexaenoic AcidsMyocardial ContractionMyocytes, CardiacAnimalsCells, CulturedPilot ProjectsRatsArachidonic AcidCytochrome P-450 Enzyme SystemDocosahexaenoic AcidsArachidonic acidCardiomyocyteContractilityCytochrome P450-derived metaboliteDocosahexaenoic acid

Identifiers

PMID41933083
PMCPMC13048951

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.