ArticleComputational and structural biotechnology journal2026
Pseudotimecascade Visualizes Gene Expression Cascade in Pseudotime Analysis.
Article in Computational and structural biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Single-cell transcriptomic technologies enable the reconstruction of dynamic biological processes such as cell development and differentiation. While existing pseudotime methods allow the analysis of temporal expression patterns, they primarily focus on individual genes, overlooking the coordinated programs that drive cellular transitions. We introduce Pseudotimecascade, a tool for visualizing and comparing multi-gene expression cascades along pseudotime. In addition, it links these cascades to biological functions by identifying stage-specific pathways. Applied to hematopoietic stem cell differentiation, Pseudotimecascade highlights regulatory hierarchies and stage-specific processes, offering a deeper understanding of gene programs that govern cell fate decisions.
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