Evidence map›Paper›PMID 41946363›Full record

ArticleCell reports. Medicine2026

Patient-derived kidney organoids recapitulate ADPKD and facilitate the identification of Rho pathway inhibitors as candidate therapeutics.

Liqiang Guo, Yujia Fan, Huixian Sun, Haoran Du, Hui Xiong, Yilin Li, Xinyan Zhang, Yong Guan, Lingtong Ren, Xuan Wang and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Liqiang GuoDepartment of Urology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Yujia FanDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Huixian SunDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Haoran DuDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Hui XiongDepartment of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Yilin LiDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Xinyan ZhangDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Yong GuanDepartment of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Lingtong RenDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Xuan WangShanghai RuiYu Biotech Co., Ltd, Shanghai, China.
Kai ChenShanghai RuiYu Biotech Co., Ltd, Shanghai, China.
Yachun AnDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Xiaohui ZhangDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Yunuo MaoDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China.
Yongfeng GongQilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Feng KongDepartment of Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China; Shandong Provincial Engineering Laboratory of Urologic Tissue Reconstruction, Jinan, China.
Jichuan QiuState Key Laboratory of Crystal Materials, Shandong University, Jinan, China.
Rui ChenShanghai RuiYu Biotech Co., Ltd, Shanghai, China.
Benkang ShiDepartment of Urology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Fan YiThe Key Laboratory of Infection and Immunity of Shandong Province, Department of Pharmacology, School of Basic Medical Sciences, Shandong University, Jinan, China. Electronic address: fanyi@sdu.edu.cn.
Huili HuDepartment of Systems Biomedicine, School of Basic Medical Sciences, Shandong University, Jinan, China; The Key Laboratory of Experimental Teratology, Ministry of Education, School of Basic Medical Sciences, Shandong University, Jinan, China; Department of Geriatric Medicine & Laboratory of Gerontology and Anti-Aging Research, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China. Electronic address: huhuili@sdu.edu.cn.
Shengtian ZhaoDepartment of Urology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China; Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China; Department of Urology, Binzhou Medical University Hospital, Binzhou, China. Electronic address: zhaoshengtian@sdu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Autosomal-dominant polycystic kidney disease (ADPKD) remains refractory to curative therapy partly due to the lack of human models that recapitulate its adult-onset nature, genetic context, and multi-segment origin. Here, we established expandable, multi-lineage adult renal organoids (MAROs) directly from surgical specimens of ADPKD patients and healthy donors, creating a three-dimensional (3D) high-content screening platform. Patient-derived MAROs faithfully reproduced hallmark cystogenic features, including elongated primary cilia, polarity disruption, and elevated Rho GTPase/planar cell polarity (PCP) signaling. Single-cell transcriptomics of PKD1/PKD2-mutant organoids revealed genotype-specific alterations. Genetic ablation of IFT88 disrupted cilia and selectively attenuated Rho/PCP activity in mutant backgrounds, supporting a cilia-dependent cyst-activating (CDCA) mechanism in cystogenesis. Microfluidic perfusion accelerated cyst expansion and amplified Rho/PCP signaling. A high-throughput drug screen identified Rho GTPase inhibitors, including ML141, as effective cyst-reducing agents across genotypes without compromising viability. Our findings establish a patient-derived organoid platform that captures ADPKD pathology and nominates Rho pathway modulation as a therapeutic strategy.

Indexed as

KidneyOrganoidsPolycystic Kidney, Autosomal Dominantrho GTP-Binding ProteinsAdultAnimalsCell PolarityCiliaFemaleHumansSignal TransductionTRPP Cation Channelsrho GTP-Binding ProteinsTRPP Cation ChannelsADPKDadult renal organoidsautosomal dominant polycystic kidney diseasedrug screeningkidney organoidmicrofluidic perfusionpatient-derived kidney organoidsPKD1PKD2planar cell polarityprimary ciliaRho GTPase signalingRho pathway

Identifiers

PMID41946363
PMCPMC13130689

What Socratic holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.