ArticleJournal of assisted reproduction and genetics2026
Pluripotency and cellular differentiation miRNAs expressed in human blastocoel fluid are associated with embryo quality.
Article in Journal of assisted reproduction and genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
purposeTo analyse the microRNAs (miRNAs) present in blastocoel fluid (BF) and assess the relationship with blastocyst quality.
methodsSeventy-two blastocysts from 57 women (25 IVF patients and 32 oocyte donors) undergoing IVF in a single private Fertility Center were included. Blastocyst quality was evaluated using Gardner´s classification. BF was collected prior to cryopreservation. MiRNA sequencing (miRNA-Seq) was employed to analyse pooled blastocoel fluid from 8 good-quality (GQ) and 8 medium/poor-quality embryos (PQ). Differential expression of miRNAs was validated in individual BF by relative quantitative real-time PCR (qRT-PCR) in 18 good-quality and 38 medium/poor-quality embryos. Target genes were identified using in silico prediction algorithms.
resultsUsing miRNA-seq we identified 85 mature and 75 immature miRNAs and selected 16 miRNAs differentially expressed in GQ and PQ embryos, with a read count exceeding 10. At the p < 0.05 level, eight miRNAs were upregulated in GQ compared to PQ embryos (miR-7107-5p, miR-4687-3p, miR-6743-5p, miR-4651, miR-6503, miR-4516, miR-4472.2, miR-409) and 8 were upregulated in PQ compared to GQ embryos (miR-6805-3p, miR-663a, miR-4438, miR-6068, miR-1182, miR-2682, miR-1908, miR-4754). In individual BF, qRT-PCR identified significantly lower expression of miR-663a, miR-7107-5p and miR-4687-3p in GQ embryos. Hatched GQ embryos had lower expression of miR-6805-3p, miR-663a and miR-4651 compared to unhatched GQ embryos. In silico predicted target genes were associated with cellular growth and differentiation, apoptosis, pluripotency, and embryonic development.
conclusionSpecific expression profiles of BF microRNAs involved in pluripotency and cellular differentiation may be associated with blastocyst quality and hatching and represent potential biomarkers for successful embryo transfer.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.