Evidence map›Paper›PMID 41955380›Full record

ArticleScience (New York, N.Y.)2026

Luminal surface proteome of the brain vasculature uncovers blood-brain barrier regulators.

Zijian Zhu, Zuzhi Jiang, Yupu Wang, Khanh Nguyen, Yuxiang Zhang, Cameron Genxuan Lian, D R Mani, Jun Zheng, Lang Ding, Shihong Max Gao and 15 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. AbioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Zijian Zhu *Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0009-0005-9104-3229
Zuzhi Jiang *Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0009-0009-1402-8235
Yupu Wang *Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-3553-0680
Khanh Nguyen *The Broad Institute of MIT and Harvard, Cambridge, MA USA.ORCID 0009-0007-0731-1097
Yuxiang Zhang *Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0009-0002-6643-2693
Cameron Genxuan LianThe Broad Institute of MIT and Harvard, Cambridge, MA USA.ORCID 0009-0007-1370-2043
D R ManiThe Broad Institute of MIT and Harvard, Cambridge, MA USA.ORCID 0000-0003-3914-133X
Jun ZhengJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-9507-8884
Lang DingJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-5365-9445
Shihong Max GaoJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0003-3238-8348
Ruyue Alps XiaJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0009-0009-7756-2494
Anne KuszpitJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Sarah LindoJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0001-5682-201X
Crystall LopezJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0009-0006-2776-7879
Catherine LindseyJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Brooke GroffJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Xinhong ChenHoward Hughes Medical Institute and Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.ORCID 0000-0003-0408-0813
Jiahui WuDepartments of Biology, Ophthalmology, and Psychology and Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA, USA.ORCID 0000-0002-1812-4488
Weiliang XiaZhiyuan College and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.ORCID 0000-0002-0256-4219
Wei LiRetinal Neurophysiology Section, National Eye Institute, Bethesda, MD, USA.ORCID 0000-0002-2897-649X
Xiaorong LiuDepartments of Biology, Ophthalmology, and Psychology and Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA, USA.ORCID 0000-0002-7655-6342
Viviana GradinaruHoward Hughes Medical Institute and Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.ORCID 0000-0001-5868-348X
Steven A CarrThe Broad Institute of MIT and Harvard, Cambridge, MA USA.ORCID 0000-0002-7203-4299
Namrata D UdeshiThe Broad Institute of MIT and Harvard, Cambridge, MA USA.ORCID 0000-0001-5312-1402
Jiefu LiJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0002-0062-4652

Funding

ProteomicsP01CA206978 · NCI · DANA-FARBER CANCER INST · PI WU, CATHERINE JU-YING · 2016 to 2025
$17.0M
Center of Excellence for High Throughput Proteogenomic CharacterizationU24CA270823 · NCI · BROAD INSTITUTE, INC. · PI STEVEN A CARR, MICHAEL A GILLETTE · 2022 to 2026
$5.3M
Proteogenomic Predictors of Recurrence in Non-small Cell Lung CancerU01CA271402 · NCI · WASHINGTON UNIVERSITY · PI STEVEN A CARR, MICHAEL A GILLETTE · 2022 to 2026
$5.0M
Center for comprehensive proteogenomic data analysisU24CA271075 · NCI · BROAD INSTITUTE, INC. · PI GAD A GETZ, Denkanikota R Mani · 2022 to 2026
$3.9M
Howard Hughes Medical InstituteNCI NIH HHS P01 CA206978NCI NIH HHS U01 CA271402NCI NIH HHS U24 CA270823NCI NIH HHS U24 CA271075
6 · The paper itself

Abstract

At the blood-tissue interface, vasculature luminal surface is critical for molecular transport, signaling transduction, and cell extravasation. Here, we present a method for proteomic profiling of the vasculature luminal surface in vivo, broadly applicable to any vertebrate. Quantitative mass spectrometry revealed the luminal surface proteome of the mouse brain vasculature and its temporal evolution from development to aging. In vivo genetic perturbation found that the arginine transporter SLC7A1 and the nitric oxide synthase NOS3 are needed for blood-brain barrier integrity in neonatal but not adult mice, whereas the hyaluronan degradation enzyme HYAL2 safeguards the barrier throughout the lifespan. By characterizing the proteomic dynamics of the vasculature luminal surface, the study links the metabolism of nitric oxide and hyaluronan to blood-brain barrier integrity.

Indexed as

Blood-Brain BarrierBrainProteomeAgingAnimalsCell Adhesion MoleculesGPI-Linked ProteinsHumansHyaluronic AcidHyaluronoglucosaminidaseMiceNitric OxideNitric Oxide Synthase Type IIIProteomicsCell Adhesion MoleculesGPI-Linked ProteinsHyal2 protein, humanHyal2 protein, mouseHyaluronic AcidHyaluronoglucosaminidaseNitric OxideNitric Oxide Synthase Type IIINos3 protein, mouseProteome

Identifiers

PMID41955380
PMCPMC13372101

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.