Evidence map›Paper›PMID 41957805›Full record

ArticleVeterinary research2026

Molecular mechanism of Tembusu virus nonstructural protein 5 antagonising RNA interference.

Meijuan Zhang, Siming Zhu, Chengguang Lu, Yafei Qin, Saisai Zhao, Xinhui Wei, Mingtian Mao, Bing Li, Xinyuan Xu, Mian Wu and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Meijuan Zhang *Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuanmingyuan West Road, Beijing, 100091, China.
Siming Zhu *College of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Chengguang LuCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Yafei QinCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Saisai ZhaoCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Xinhui WeiCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Mingtian MaoCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Bing LiInstitute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuanmingyuan West Road, Beijing, 100091, China.
Xinyuan XuCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Mian WuCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Huihui LiInstitute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuanmingyuan West Road, Beijing, 100091, China.
Zhuo ZhangInstitute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuanmingyuan West Road, Beijing, 100091, China.
Youxiang DiaoCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China.
Dalin HeCollege of Veterinary Medicine, Shandong Agricultural University, No. 7, Panhe Street, Tai'an, 271001, Shandong, China. dlhe@sdau.edu.cn.
Tang YiInstitute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuanmingyuan West Road, Beijing, 100091, China. tyck288@163.com.

Funding

China Agriculture Research System of MOF and MARA CARS-42-19National Key Research and Development Program for Young Scientists 2022YFD1801900National Natural Science Foundation of China 32172845Natural Science Foundation of Shandong Province ZR2020MC183State-sponsored Postdoctoral Research Program GZC20231503The Project Charter for the Shandong Provincial Key Research and Development Program in Agricultural Breeding Engineering 2024LZGC021-04
6 · The paper itself

Abstract

Tembusu virus (TMUV), a member of the genus Orthoflavivirus within the family Flaviviridae, is an emerging pathogen that severely impacts waterfowl, causing decreased egg production in laying ducks and neurological symptoms in ducklings and growing birds. RNA interference (RNAi) is a conserved post-transcriptional regulatory mechanism mediated by small non-coding RNAs in eukaryotic cells, while many viruses can antagonise host RNAi-mediated antiviral immunity by encoding viral suppressors of RNAi (VSRs). In this study, we demonstrated that TMUV antagonises the RNAi pathway. Using enhanced green fluorescent protein (EGFP)-based RNAi reporter systems in both HEK293T and Drosophila S2 cells, we identified the TMUV nonstructural protein 5 (NS5) as a potent VSR. Furthermore, we found that NS5 not only interacts with DEAD-box helicase 3 X-linked (DDX3X), a key protein in the RNAi pathway, but also binds directly to double-stranded RNA (dsRNA). In summary, our findings indicated that TMUV NS5 can act as a VSR in vitro, thereby providing a theoretical foundation for the development of antiviral therapeutics.

Indexed as

FlavivirusFlavivirus InfectionsRNA InterferenceViral Nonstructural ProteinsAnimalsCell LineHEK293 CellsHumansNS5 protein, flavivirusViral Nonstructural Proteinsinnate immunitynonstructural Protein 5RNA interferenceTembusu virusviral suppressor of RNAi

Identifiers

PMID41957805
PMCPMC13154847

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.