Evidence map›Paper›PMID 41957835›Full record

ArticleJournal of the science of food and agriculture2026

Chitosan-induced modulation of secondary metabolism and stress tolerance in Salvia rosmarinus under combined drought and heat stress.

Inês Mansinhos, Sandra Gonçalves, Raquel Rodríguez-Solana, Efrén Pérez-Santín, María I Fernández-Marín, Emma Cantos-Villar, Anabela Romano

Abstract read
In one paragraph

Article in Journal of the science of food and agriculture, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Inês MansinhosMED - Mediterranean Institute for Agriculture, Environment and Development, and CHANGE - Global Change and Sustainability Institute, Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, Faro, Portugal.ORCID https://orcid.org/0000-0001-9632-8876
Sandra GonçalvesMED - Mediterranean Institute for Agriculture, Environment and Development, and CHANGE - Global Change and Sustainability Institute, Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, Faro, Portugal.ORCID https://orcid.org/0000-0002-3038-4434
Raquel Rodríguez-SolanaMED - Mediterranean Institute for Agriculture, Environment and Development, and CHANGE - Global Change and Sustainability Institute, Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, Faro, Portugal.ORCID https://orcid.org/0000-0003-4204-7387
Efrén Pérez-SantínEscuela Superior de Ingeniería y Tecnología (ESIT), Universidad Internacional de La Rioja - UNIR, Logroño, Spain.ORCID https://orcid.org/0000-0002-1484-3127
María I Fernández-MarínDepartment of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Rancho de La Merced Center, Cádiz, Spain.
Emma Cantos-VillarDepartment of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Rancho de La Merced Center, Cádiz, Spain.ORCID https://orcid.org/0000-0001-8560-8821
Anabela RomanoMED - Mediterranean Institute for Agriculture, Environment and Development, and CHANGE - Global Change and Sustainability Institute, Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, Faro, Portugal.ORCID https://orcid.org/0000-0002-7204-7428

Funding

Agencia Estatal de Investigación (AEI) RYC2022-036888-IEuropean Social Fund Plus (FSE+)Ministerio de Ciencia e Innovación (MCIN)/AEI/10.13039/501100011033Raquel Rodríguez Solana RYC2022-036888-I
6 · The paper itself

Abstract

backgroundAbiotic stresses, particularly drought and elevated temperatures, negatively affect plant physiological and metabolic processes. This study investigated the effects of drought, heat, and combined stress on Salvia rosmarinus (rosemary), and evaluated foliar-applied chitosan as a biostimulant. After 3 weeks, photosynthetic pigments, osmoprotectants (proline, soluble sugars), oxidative stress indicators (hydrogen peroxide and lipid peroxidation), phenolic compounds, essential oils, and antioxidant activity were evaluated. A natural deep eutectic solvent (NADES) was used to enhance phenolic extraction sustainably.

resultsCombined stress intensified oxidative damage and reduced photosynthetic pigments, while elevated osmoprotectants and chlorophyll a/b ratio reflected adaptive responses. Chitosan improved pigment retention, boosted sugar levels, and mitigated oxidative damage. Stress-exposed, chitosan-treated plants showed increased rosmarinic acid and key monoterpenes (camphor, α-pinene, 1,8-cineole), indicating enhanced secondary metabolism. Antioxidant assays confirmed superior scavenging and reducing activities in chitosan-treated plants under stress.

conclusionChitosan modulated physiological and metabolic responses in Salvia rosmarinus, improving resilience and phytochemical profile under abiotic stress. This is the first report demonstrating foliar-applied chitosan's effectiveness in Salvia rosmarinus facing drought and heat. © 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Indexed as

ChitosanRosmarinusAntioxidantsDrought ResistanceDroughtsHot TemperatureOxidative StressPhenolsPlant LeavesSecondary MetabolismStress, PhysiologicalAntioxidantsChitosanPhenolsbiostimulationGC‐MSHPLCphenolicsRosmarinus officinalisterpenoids

Identifiers

PMID41957835
PMCPMC13258217

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.