Evidence map›Paper›PMID 41959495›Full record

ArticlebioRxiv : the preprint server for biology2026

In vitro reconstitution of vertebrate Sonic Hedgehog protein cholesterolysis.

Dayton C Seidel, Andrew G Wagner, John L Pezzullo, Katherine A Thayer, Seth Beadle, Margot L Olejarczyk, José-Luis Giner, Brian P Callahan

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Dayton C SeidelDepartment of Chemistry, Binghamton University, State University of New York, Binghamton, New York 13902, United States.
Andrew G WagnerDepartment of Chemistry, Binghamton University, State University of New York, Binghamton, New York 13902, United States.
John L PezzulloDepartment of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, New York 13210, United States.
Katherine A ThayerDepartment of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, New York 13210, United States.
Seth BeadleDepartment of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, New York 13210, United States.
Margot L OlejarczykDepartment of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, New York 13210, United States.
José-Luis GinerDepartment of Chemistry, State University of New York College of Environmental Science and Forestry, Syracuse, New York 13210, United States.
Brian P CallahanDepartment of Chemistry, Binghamton University, State University of New York, Binghamton, New York 13902, United States.

Funding

A 800 MHz Nuclear Magnetic Resonance Spectrometer in Support of Life Science ReseS10OD012254 · OD · UPSTATE MEDICAL UNIVERSITY · PI WILKENS, STEPHAN · 2013 to 2013
$2.0M
Sterol Probes of Hedgehog Protein ProcessingR15GM143714 · NIGMS · COLLEGE OF ENVIRONMENTAL SCI & FORESTRY · PI GINER, JOSE L · 2021 to 2021
$446k
Discovery and anti-tumor activity of the first antagonists targeting human Sonic Hedgehog autoprocessingR21CA282857 · NCI · STATE UNIVERSITY OF NY,BINGHAMTON · PI CALLAHAN, BRIAN PATRICK · 2024 to 2025
$402k
NCI NIH HHS R21 CA282857NIGMS NIH HHS R15 GM143714NIH HHS S10 OD012254
6 · The paper itself

Abstract

Extracellular secretion of the oncogenic sonic hedgehog signaling ligand is contingent on its release from a precursor protein through peptide bond cholesterolysis, mediated by the hedgehog C-terminal domain, SHhC. In this work, we describe the in vitro reconstitution of cholesterolysis activity for SHhC domains from vertebrate model organisms,

Identifiers

PMID41959495
PMCPMC13060885

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.