Evidence mapPaperPMID 41963923Full record

ArticleJournal of biomedical science2026

Many but not all pathogen-associated molecular patterns aggravate neurogenic heterotopic ossification after spinal cord injury.

Selwin G Samuel, Hsu-Wen Tseng, Bastien Rival, Valérie Barbier, Kavita Bisht, Marjorie Salga, Shrutika M Mate, Whitney Fleming, François Genêt, Sébastien Banzet and 3 more

Abstract read
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Article in Journal of biomedical science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Selwin G SamuelMater Research Institute-The University of Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba, QLD, 4102, Australia.
Hsu-Wen TsengMater Research Institute-The University of Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba, QLD, 4102, Australia.
Bastien RivalInstitut de Recherche Biomédicale des Armées (IRBA), INSERM UMR-MD 1197, 1, Rue du Lieutenant Raoul Batany, 92140, Clamart, France.
Valérie BarbierMater Research Institute-The University of Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba, QLD, 4102, Australia.
Kavita BishtMater Research Institute-The University of Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba, QLD, 4102, Australia.
Marjorie SalgaUPOH (Unité Péri Opératoire du Handicap), Physical and Rehabilitation Medicine Department, Raymond-Poincaré Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Garches, France.
Shrutika M MateMater Research Institute-The University of Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba, QLD, 4102, Australia.
Whitney FlemingMater Research Institute-The University of Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba, QLD, 4102, Australia.
François GenêtUPOH (Unité Péri Opératoire du Handicap), Physical and Rehabilitation Medicine Department, Raymond-Poincaré Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Garches, France.
Sébastien BanzetInstitut de Recherche Biomédicale des Armées (IRBA), INSERM UMR-MD 1197, 1, Rue du Lieutenant Raoul Batany, 92140, Clamart, France.
Jean-Pierre LévesqueMater Research Institute-The University of Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba, QLD, 4102, Australia. jp.levesque@mater.uq.edu.au.
Dorothée GirardInstitut de Recherche Biomédicale des Armées (IRBA), INSERM UMR-MD 1197, 1, Rue du Lieutenant Raoul Batany, 92140, Clamart, France. dorothee.girard@inserm.fr.
Kylie A AlexanderMater Research Institute-The University of Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba, QLD, 4102, Australia. kylie.alexander@mater.uq.edu.au.

Funding

Congressionally Approved Spinal Cord Injury Research Program of the US Department of Defense W81XWH2110560
6 · The paper itself

Abstract

backgroundNeurogenic heterotopic ossifications (NHOs) are heterotopic bones that develop in periarticular muscles after traumatic brain (TBI) and spinal cord injuries (SCI). The mechanisms leading to NHO are incompletely understood and the only effective treatment to-date remains surgical resection. We previously established that several inflammatory pathways drive NHO pathogenesis in injured muscles in a mouse model of NHO and in humans. We also demonstrated a functional association between gram-negative bacterial infections and NHO development via lipopolysaccharide (LPS), a pathogen associated molecular pattern (PAMP), which exacerbated NHO in a Toll-like receptor-4 (TLR4)-dependent manner in mice.

methodsUsing our mouse model of NHO induced by SCI and muscle injury in mice, we tested the effect of a large array of purified PAMPs post-surgery to mimic fungal, viral and bacterial infections and measured NHO bone volumes by micro-computerized tomography (microCT). The effect of PAMPs was also tested in vitro on human muscle progenitors and monocyte/macrophage populations.

resultsMuscle progenitors and monocyte/macrophage populations from humans and mice express numerous pattern recognition receptors. In mice, numerous PAMPs produced by bacteria, viruses and fungi exacerbated NHO formation, and the majority of these PAMPs indirectly stimulated fibro-adipogenic progenitor (FAP) calcium mineralization in vitro via macrophages. Likewise, in humans, some PAMPs, particularly those binding to TLR2, directly and indirectly increased the calcium mineralization and osteogenic differentiation of human FAPs isolated from muscles surrounding human NHO. Finally, we established that the indirect stimulation of human FAP mineralization was mediated by inflammatory cytokines IL-1 and oncostatin M secreted by monocytes in response to PAMPs. Overall, our findings suggest that numerous types of infection have the potential to exacerbate NHO development and further highlight the role of oncostatin M and IL-1 signaling pathways in NHO pathophysiology.

Indexed as

Ossification, HeterotopicSpinal Cord InjuriesAnimalsDisease Models, AnimalHumansMiceBone formationInfectionInflammationInterleukin-1Neurogenic heterotopic ossificationOncostatin MPathogen-associated molecular patternsPattern recognition receptorsSpinal cord injury

Identifiers

PMID41963923
PMCPMC13067622

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.