Evidence map›Paper›PMID 41964260›Full record

ArticleMolecular plant pathology2026

Functional Validation of the Melon Fom-1 Gene, Controlling Resistance to Fusarium oxysporum Races 0 and 2, by CRISPR/Cas9 Mutagenesis.

Balasubramanian Muthusamy, Shahar Nizan, Amalia Bar-Ziv, Rafael Perl-Treves

Abstract read
In one paragraph

Article in Molecular plant pathology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Balasubramanian MuthusamyThe Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel.ORCID 0009-0005-9233-9822
Shahar NizanThe Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel.ORCID 0009-0003-0069-2334
Amalia Bar-ZivThe Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel.
Rafael Perl-TrevesThe Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel.ORCID 0000-0003-0294-6900

Funding

Chief Scientist grant for Biotechnology, Ministry of Agriculture, Israel 873-0074-11Israel Science Foundation 1137/16
6 · The paper itself

Abstract

Functional validation of NLR genes is critical for confirming their specific roles and developing durable disease-resistant crops. The Fom-1 gene of Cucumis melo, controlling resistance to races 0 and 2 of F. oxysporum f. sp. melonis (FOM), had been identified by map-based cloning as MELO3C022146, which encodes a TIR-NBS-LRR (TNL) protein. It resides in a head-to-head orientation adjacent to another TNL gene, Prv, controlling resistance to papaya ring spot virus (PRSV). In this study we validated the function of Fom-1 in mediating FOM resistance by applying CRISPR/Cas9 editing to the resistant cultivar, Védrantais. Two gRNAs were designed to target exons 1 and 2, respectively, and mutations were introduced at both target sites, resulting in truncated open reading frames in both alleles. Inoculation assays of T

Indexed as

CRISPR-Cas SystemsCucumis meloCucurbitaceaeDisease ResistanceFusariumGenes, PlantMutagenesisPlant DiseasesPlant ProteinsBase SequencePlant ProteinsCRISPR/Cas9Cucumis meloFusarium oxysporum f. sp. melonismelonNLR generesistance gene

Identifiers

PMID41964260
PMCPMC13069143

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.