Evidence map›Paper›PMID 41965806›Full record

ArticleVirology journal2026

Detection of dengue virus serotypes in Aedes larvae: a multi-location study from Bhubaneswar, India, suggestive of vertical transmission.

Aditi Pal, Swatismita Priyadarsini, Jyotsnamayee Sabat, Ira Praharaj, Arun Kumar Pradhan, Mahesh Chandra Sahu, Sanghamitra Pati

Abstract read
In one paragraph

Article in Virology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Aditi PalCentre for Biotechnology, Siksha 'O' Anusandhan Deemed to be University, Kalinga Nagar, Bhubaneswar, 751003, Odisha, India.
Swatismita PriyadarsiniVRDL, ICMR-Regional Medical Research Centre, Nalco Nagar, Chandrasekharpur, Bhubaneswar, 751023, Odisha, India.
Jyotsnamayee SabatVRDL, ICMR-Regional Medical Research Centre, Nalco Nagar, Chandrasekharpur, Bhubaneswar, 751023, Odisha, India.
Ira PraharajVRDL, ICMR-Regional Medical Research Centre, Nalco Nagar, Chandrasekharpur, Bhubaneswar, 751023, Odisha, India.
Arun Kumar PradhanCentre for Biotechnology, Siksha 'O' Anusandhan Deemed to be University, Kalinga Nagar, Bhubaneswar, 751003, Odisha, India. arunpradhan@soa.ac.in.
Mahesh Chandra SahuDivision of Microbiology, ICMR-Regional Medical Research Centre, NALCO Nagar, Chandrasekharpur, Bhubaneswar, 751023, Odisha, India. mchsahu@gmail.com.
Sanghamitra PatiDepartment of Health Research, Indian Council of Medical Research, New Delhi, 110029, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dengue virus (DENV), primarily spread by Aedes mosquitoes, remains a significant public health concern in India. This study focused on Bhubaneswar, Odisha, India aiming to detect DENV serotypes in Aedes larvae to gain early insights into viral circulation. A total of 8,257 larvae were collected from 60 locations, pooled (n = 390) and screened for dengue using real-time-PCR (pan-dengue assay). Serotyping was carried out using serotype-specific RT-PCR targeting DENV-1 to DENV-4. Overall, 60 pools tested positive for DENV. DENV-1 emerged as the dominant serotype across both winter and rainy seasons. DENV-2 appeared only during the rainy season, often in co-infections with DENV-1 in areas such as Bhagabatipur, Katani, and Dashpur. Higher viral loads were detected in Dashpur, BDA Colony, and Acharya Vihar. Persistent detection near locations like Kalinga Hospital and evidence of vertical (transovarial) transmission further emphasize the ongoing risk. The emergence of adult Aedes from larvae confirmed vector species identity. These findings underscore the value of larval molecular surveillance in identifying transmission hotspots and timing interventions. Integrating such data with public health efforts can significantly enhance dengue outbreak prediction and vector control in urban settings.

Indexed as

AedesDengueDengue VirusInfectious Disease Transmission, VerticalMosquito VectorsAnimalsIndiaLarvaReal-Time Polymerase Chain ReactionSeasonsSerogroupSerotypingViral LoadAedes aegyptiAedes albopictusAedes larvaeDENVRT-PCRViral RNA

Identifiers

PMID41965806
PMCPMC13214227

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.