Evidence map›Paper›PMID 41968245›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

PCR Typing and Genome Sequencing of Aspergillus fumigatus.

Hiroki Takahashi, Saho Shibata, Momotaka Uchida

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hiroki TakahashiMedical Mycology Research Center, Chiba University, Chiba, Japan. hiroki.takahashi@chiba-u.jp.
Saho ShibataMedical Mycology Research Center, Chiba University, Chiba, Japan.
Momotaka UchidaMedical Mycology Research Center, Chiba University, Chiba, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aspergillus fumigatus is a major opportunistic fungal pathogen, presenting a serious health risk, particularly to immunocompromised individuals. Comprehensive genomic analysis of both clinical and environmental isolates provides valuable insights into its population structure, antifungal resistance mechanisms, and evolutionary dynamics. This chapter outlines protocols for DNA extraction and typing PCR, including a step-by-step workflow for high-resolution genomic analysis of A. fumigatus using short-read sequencing data. This protocol enables researchers to perform reliable variant analysis and phylogenomic reconstruction, offering a reproducible protocol for genomic studies in A. fumigatus and related fungal species.

Indexed as

Aspergillus fumigatusGenome, FungalPolymerase Chain ReactionWhole Genome SequencingDNA, FungalGenomicsHumansPhylogenyDNA, FungalAspergillus fumigatusBioinformatics pipelinePCR typingPhylogenomicsVariant analysisWhole-genome sequencing

Identifiers

PMID41968245

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.