Evidence map›Paper›PMID 41968250›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Electrophoretic Mobility Shift Assay in Aspergillus fumigatus.

Vijendra Arya, Sanjoy Paul, W Scott Moye-Rowley

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Vijendra AryaDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Sanjoy PaulDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
W Scott Moye-RowleyDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA. scott-moye-rowley@uiowa.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

One of the classic techniques to allow detection of direct DNA-binding by sequence-specific transcription factors is the electrophoretic mobility shift assay (EMSA). EMSA takes advantage of the reduced mobility of a protein:DNA complex during gel electrophoresis compared to the naked DNA alone. This rapid and straightforward method has been used to identify DNA target elements in organisms ranging from bacteria to humans. Here we describe its use with a focus on the AtrR transcription factor from the filamentous fungus Aspergillus fumigatus.

Indexed as

Aspergillus fumigatusElectrophoretic Mobility Shift AssayDNA-Binding ProteinsDNA, FungalFungal ProteinsProtein BindingTranscription FactorsDNA-Binding ProteinsDNA, FungalFungal ProteinsTranscription FactorsAspergillus fumigatusElectrophoretic mobility shift assayEMSA

Identifiers

PMID41968250

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.