Evidence map›Paper›PMID 41980460›Full record

ArticleRedox biology2026

Dual targeting of NCF1 and NLRP3 by roburic acid orchestrates redox homeostasis and inhibits macrophage death in septic lung injury.

Honghao Song, Lei Peng, Yutong Yang, Kaiqiang Sun, Yan Chu, Mengqiu Deng, Xiaoyan Meng, Huawei Wei, Jiao Cai, Yi Liu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Honghao SongDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China.
Lei PengDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China.
Yutong YangDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China.
Kaiqiang SunDepartment of Orthopedic Surgery, Changzheng Hospital, Navy Medical University, Shanghai, 200003, People's Republic of China.
Yan ChuDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China.
Mengqiu DengDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China.
Xiaoyan MengDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China.
Huawei WeiDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China.
Jiao CaiDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China. Electronic address: caijiaosmmu@163.com.
Yi LiuFaculty of Anesthesiology, Changhai Hospital, Naval Military Medical University, Shanghai, 200433, People's Republic of China. Electronic address: ziboliuyi@yeah.net.
Hongbin YuanDepartment of Anesthesiology, Changzheng Hospital, Naval Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China. Electronic address: jfjczyy@aliyun.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sepsis-associated acute lung injury (ALI) is characterized by excessive inflammation and macrophage death, yet precise therapeutic targets remain limited. Single-cell sequencing analysis indicates that, with progression of sepsis-induced lung injury, macrophages exhibit diverse cell-death programs with prominent enrichment of pyroptosis-related signatures. Here, we identify Roburic acid (RBA) as a potent inhibitor of septic ALI and elucidate its mechanism using a nanoparticle delivery system (RBA-NPs). We demonstrate that RBA-NPs significantly attenuate lung injury through a bioactive lipid compound library screening and improve survival in cecal ligation and puncture (CLP)-induced sepsis models. Mechanistically, using chemical proteomics and cellular thermal shift assays, we identify NLRP3 and NCF1 as direct intracellular targets of RBA. Primarily, RBA interacts with the NACHT domain of NLRP3 to directly block inflammasome assembly and pyroptosis. Furthermore, RBA binds to NCF1 to inhibit NADPH oxidase 2 assembly; this restores redox homeostasis, which not only reinforces the suppression of pyroptosis but also confers additional protection by inhibiting lipid peroxidation-mediated ferroptosis. Our study reveals a comprehensive therapeutic strategy where RBA targets the NLRP3 inflammasome while coordinating redox homeostasis via NCF1 to resolve septic lung injury.

Indexed as

Acute Lung InjuryMacrophagesNADPH OxidasesNLR Family, Pyrin Domain-Containing 3 ProteinSepsisAnimalsChrysenesDisease Models, AnimalHomeostasisHumansInflammasomesMiceOxidation-ReductionPyroptosisChrysenesInflammasomesNADPH Oxidasesneutrophil cytosolic factor 1NLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mouseroburic acidBioactive lipid compound libraryMacrophage ferroptosisMacrophage pyroptosisOxidative stressRoburic acidSepsis-induced lung injurySingle-cell sequencing

Identifiers

PMID41980460
PMCPMC13092860

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.