Evidence map›Paper›PMID 41989617›Full record

ArticleMolecular biology reports2026

Melatonin inhibits malignant biological behaviors in carboplatin-resistant ovarian cancer cells: elucidating the potential molecular mechanisms.

Xiaoying Li, Xiaoyu Che, Yanhong Bi

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Xiaoying Li *Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Shenyang, China.
Xiaoyu Che *Department of Research, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.
Yanhong BiDepartment of Research, Affiliated Zhongshan Hospital of Dalian University, Dalian, China. cmuyhbi@163.com.

Funding

the Liaoning Provincial Natural Science Foundation of China 2025-BS-0559
6 · The paper itself

Abstract

backgroundThe role of melatonin (MLT) in modulating malignant phenotypes of carboplatin (CBP)-resistant ovarian cancer (OC) cells remains unexplored. This study therefore aimed to investigate effects of MLT on malignant biological behaviors in CBP-resistant OC cells and to preliminarily delineate underlying molecular mechanisms.

methodsCBP-resistant A2780 and SKOV3 cell lines were first established, and their resistance index (RI) was determined by CCK-8 assay. Subsequently, effects of MLT were systematically evaluated: cell proliferation was assessed by CCK-8 assay; cell cycle distribution and apoptosis were analyzed by flow cytometry; and cell invasion and migration capabilities were examined using Transwell chambers. The potential mechanism by which MLT modulates malignant biological behaviors in CBP-resistant OC cells was elucidated through mRNA sequencing and bioinformatics analyses.

resultsCBP-resistant OC cell lines were successfully established. We demonstrated that MLT effectively suppressed key malignant phenotypes of CBP-resistant OC cells, including proliferation, cell cycle progression, invasion, and migration, while promoting apoptosis (P < 0.05). Transcriptomic analyses demonstrated that MLT upregulated 548 genes and downregulated 119 genes in A2780/CBP cells, and upregulated 70 genes and downregulated 232 genes in SKOV3/CBP cells. Notably, a common set of 26 differentially expressed genes (DEGs) was consistently identified in both resistant cell lines. These shared DEGs were significantly enriched in the GO term “negative regulation of canonical Wnt signaling pathway” and KEGG pathway “amphetamine addiction”.

conclusionsMLT inhibited malignant biological behaviors of CBP-resistant OC cells. The underlying mechanism likely involved the “negative regulation of canonical Wnt signaling pathway” (GO term) and “amphetamine addiction” (KEGG pathway).

Indexed as

CarboplatinDrug Resistance, NeoplasmMelatoninOvarian NeoplasmsApoptosisCell CycleCell Line, TumorCell MovementCell ProliferationFemaleGene Expression Regulation, NeoplasticHumansWnt Signaling PathwayCarboplatinMelatoninCanonical Wnt signaling pathwayCarboplatinMelatoninMRNA sequencingOvarian cancer

Identifiers

PMID41989617

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.