Evidence map›Paper›PMID 41990257›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Mitochondria and Neuromast Tagging With Fluorescent Gallium-Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging.

Megan J Green, Michael W Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon-Tamarit, Rory L Arrowsmith, Charareh Pourzand, Gabriele Kociock-Kohn and 6 more

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Megan J GreenDepartment of Chemistry, University of Bath, Claverton Down, Bath, UK.
Michael W JonesChemistry Research Laboratory, University of Oxford, Oxford, UK.
Merissa SaleemDepartment of Life Sciences, University of Bath, Claverton Down, Bath, UK.
Haobo GeDepartment of Chemistry, University of Bath, Claverton Down, Bath, UK.
Melita TvardauskaiteDepartment of Life Sciences, University of Bath, Claverton Down, Bath, UK.
Julia DudzicDepartment of Life Sciences, University of Bath, Claverton Down, Bath, UK.
Fernando Cortezon-TamaritDepartment of Chemistry, University of Bath, Claverton Down, Bath, UK.
Rory L ArrowsmithDepartment of Chemistry, University of Bath, Claverton Down, Bath, UK.
Charareh PourzandDepartment of Life Sciences, University of Bath, Claverton Down, Bath, UK.
Gabriele Kociock-KohnDepartment of Chemistry, University of Bath, Claverton Down, Bath, UK.
Nicholas H ReesChemistry Research Laboratory, University of Oxford, Oxford, UK.
Jonathan R DilworthChemistry Research Laboratory, University of Oxford, Oxford, UK.
Stephen FaulknerChemistry Research Laboratory, University of Oxford, Oxford, UK.
Stanley W BotchwayCentral Laser Facility, Rutherford Appleton Laboratory, Research Complex at Harwell, STFC, Didcot, UK.
David GurevichDepartment of Life Sciences, University of Bath, Claverton Down, Bath, UK.
Sofia I PascuDepartment of Chemistry, University of Bath, Claverton Down, Bath, UK.ORCID https://orcid.org/0000-0001-6385-4650

Funding

aid of the EPSRC Dynamic Reaction Monitoring Facility at the University of Bath EP/P001475/1BBSRC BB/W019655/1BBSRC EP/L016354/1BBSRC EPSRC IAA+ EP/X525650/1ERC Consolidator Grant O2Sense 617107ERC PoC Tools-To-Sense 963937Research Complex at Harwell, the experiment grants 20230032Training in Sustainable Chemical Technologies EP/G03768X/1Welcome Funding Agency 220188/A/20/Z
6 · The paper itself

Abstract

We report on the synthesis and imaging-based investigations of fluorescent thiosemicarbazone-BODIPY conjugates and their gallium(III), indium(III), and iron(III) complexes, and their cellular and in vivo distribution using multiphoton fluorescence lifetime imaging microscopy (MP-FLIM) in living cancer cells and live zebrafish. A panel of novel tridentate thiosemicarbazone ligands and their corresponding lipophilic and monocationic complexes with 1:2 metal-to-ligand stoichiometry was synthesized. These compounds are structurally related to clinically relevant thiosemicarbazone-based systems with imaging and diagnostic applications. Comprehensive analytical characterization confirmed these probes' identity, while their complex solution behavior was evaluated under biologically relevant conditions. Cellular uptake and subcellular distribution were investigated in HeLa and PC-3 cells using correlated confocal microscopy and MP-FLIM, revealing preferential mitochondrial localization for selected gallium complexes. In vivo imaging in zebrafish further unraveled the biodistribution of these fluorescent metal complexes, including selective labelling of neuromast structures. The combined in vitro and in vivo imaging data provide direct insight into the speciation, stability, and spatial distribution of thiosemicarbazone metal complexes in complex biological systems. This work establishes new fluorescent thiosemicarbazones as versatile platforms for studying metal complex behavior and biodistribution by advanced optical imaging techniques, opening new opportunities for advanced development of new theranostic applications.

Indexed as

BODIPY conjugatescellular uptakecorrelated confocal microscopymetal‐thiosemicarbazonesMP FLIMzebrafish imaging

Identifiers

PMID41990257
PMCPMC13335005

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.