Evidence map›Paper›PMID 41998164›Full record

ArticleNature biomedical engineering2026

Three-dimensional quantitative tissue clearing reveals differences in osteovascular niche of aged and young human mesenchymal stromal cells.

Nelson Tsz Long Chu, Ostap Dregval, Yu-Wei Chang, Emil Kriukov, Xin Tian, Xin Liu, Dana Trompet, Misty Shuo Zhang, Lei Li, Zhong Li and 10 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature biomedical engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Nelson Tsz Long ChuCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Ostap DregvalCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.ORCID http://orcid.org/0000-0002-9939-6492
Yu-Wei ChangDepartment of Physics, Gothenburg University, Gothenburg, Sweden.
Emil KriukovDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Xin TianCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Xin LiuCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Dana TrompetCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.ORCID http://orcid.org/0000-0001-9472-6184
Misty Shuo ZhangDepartment of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Lei LiCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Zhong LiCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Emiliano Gomez RuizDepartment of Physics, Gothenburg University, Gothenburg, Sweden.
Joana B PereiraDepartment of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.ORCID http://orcid.org/0000-0002-4604-2711
Mats BrittbergCartilage Research Unit, Department of Orthopedics, University of Gothenburg, Gothenburg, Sweden.
Björn BareniusDepartment of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden.
Lars SävendahlDepartment of Women and Children's Health, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.ORCID http://orcid.org/0000-0003-1067-4976
Ralf H AdamsDepartment of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, Münster, Germany.ORCID http://orcid.org/0000-0003-3031-7677
Inger GjertssonDepartment of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.ORCID http://orcid.org/0000-0002-9301-4844
Claes OhlssonCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Giovanni VolpeDepartment of Physics, Gothenburg University, Gothenburg, Sweden.ORCID http://orcid.org/0000-0001-5057-1846
Andrei S ChaginCentre for Bone and Arthritis Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. andrei.chagin@gu.se.ORCID http://orcid.org/0000-0002-2696-5850

Funding

IngaBritt och Arne Lundbergs Forskningsstiftelse (Ingabritt and Arne Lundberg Research Foundation) LU2025-0079Novo Nordisk Fonden (Novo Nordisk Foundation) NNF25OC0105090Vetenskapsrådet (Swedish Research Council) 2025-03154
6 · The paper itself

Abstract

Human bone marrow mesenchymal stromal/stem cells (BM-MSCs) are widely used in clinical trials and tissue engineering, yet their native microenvironment remains poorly understood. Here we introduce a tissue-clearing protocol, DeepBone, for human bones and integrate it with simultaneous mRNA and protein detection. Using this protocol, we spatially map BM-MSCs relative to key bone microenvironment components, including human blood capillaries, adipocytes, sinusoids and bony trabeculae. Quantitative analysis reveals that the native microenvironment of human BM-MSCs in young bone is enriched in vasculature, sinusoids, bone matrix and adipocytes. In contrast, in aged bone, BM-MSCs show no preferential association with bone or adipocytes. Proliferative BM-MSCs are predominantly found along blood vessels. Moreover, we identify a specialized microenvironment for BM-MSCs in young bone, characterized by sinusoids coiled around trabeculae and enriched by R-type vessels. These findings provide insights into the native niches of BM-MSCs, offering a foundation for the development of tissue engineering strategies that mimic their physiological context.

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.