Evidence mapPaperPMID 41999751Full record

ArticleCell reports. Medicine2026

Combined multi-omics and multi-spectral profiling of plasma extracellular vesicles reveals liquid biopsy biomarkers for glioma diagnosis.

Stephen David Robinson, Biniam Tsegay Haile, Matthew Reily-Bell, Olivia Iwanowytsch, Siobhan Palmer, Dorte Schou Nørøxe, Panagiota S Filippou, Joanna Renaut, Alan Lazarus, Georgios Antoniou and 25 more

Abstract read
In one paragraph

Article in Cell reports. Medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

35 authors.

Stephen David RobinsonDepartment of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK; Sussex Cancer Centre, University Hospitals Sussex NHS Foundation Trust, Brighton BN2 5BE, UK. Electronic address: s.d.robinson@sussex.ac.uk.
Biniam Tsegay HaileBioinformatics Laboratory, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
Matthew Reily-BellDunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand.
Olivia IwanowytschNanoOmics Lab, Centre for Nanotechnology in Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PL, UK.
Siobhan PalmerDxcover Ltd., Glasgow G33 1AP, UK; WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow G1 1XL, UK.
Dorte Schou NørøxeDanish Comprehensive Cancer Center Brain Tumor Centre, 2100 Copenhagen, Denmark; Department of Oncology, University Hospital of Copenhagen, 2100 Copenhagen, Denmark.
Panagiota S FilippouSchool of Health & Life Sciences, Teesside University, Middlesbrough TS1 3BX, UK; National Horizons Centre, Teesside University, Darlington DL1 1HG, UK; School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.
Joanna RenautBioinformatics Laboratory, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
Alan LazarusDxcover Ltd., Glasgow G33 1AP, UK.
Georgios AntoniouDxcover Ltd., Glasgow G33 1AP, UK.
Mark SamuelsInternational Oncology Institute, The First Affiliated Hospital of Zhejiang Chinese Medical University, Oncology Department of the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China; Department of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
Viviana VellaDepartment of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
Chrysa FilippopoulouInternational Oncology Institute, The First Affiliated Hospital of Zhejiang Chinese Medical University, Oncology Department of the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China.
William JonesDepartment of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
Josephine JungDepartment of Neurosurgery, Kings College Hospital NHS Foundation Trust, London SE5 9RS, UK.
Xiaoou LiDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.
Nan JiDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.
Yang ZhangDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.
Aleena AzamDepartment of Neurosurgery, University Hospital of Copenhagen, 2100 Copenhagen, Denmark.
Jane Skjoeth-RasmussenDepartment of Neurosurgery, University Hospital of Copenhagen, 2100 Copenhagen, Denmark.
Ulrik LassenDanish Comprehensive Cancer Center Brain Tumor Centre, 2100 Copenhagen, Denmark; Department of Oncology, University Hospital of Copenhagen, 2100 Copenhagen, Denmark.
Adriana SaraivaDunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand.
Ahmad TahaDunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand.
Tania SlatterDunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand.
Greg JonesDunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand.
Rajesh KatareDunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand.
Holly J ButlerDxcover Ltd., Glasgow G33 1AP, UK.
Matthew J BakerDxcover Ltd., Glasgow G33 1AP, UK; School of Medicine, University of Central Lancashire, Preston PR1 7BH, UK.
Marilena HadjidemetriouNanoOmics Lab, Centre for Nanotechnology in Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PL, UK.
Duncan GilbertSussex Cancer Centre, University Hospitals Sussex NHS Foundation Trust, Brighton BN2 5BE, UK; MRC Clinical Trials Unit, University College London, London WC1V 6LJ, UK.
Benjamin TowlerDepartment of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
Keyoumars AshkanDepartment of Neurosurgery, Kings College Hospital NHS Foundation Trust, London SE5 9RS, UK.
Giles CritchleyDunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand; Department of Neurosurgery, University Hospitals Sussex NHS Foundation Trust, Brighton BN2 5BE, UK.
Frances M G PearlBioinformatics Laboratory, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
Georgios GiamasInternational Oncology Institute, The First Affiliated Hospital of Zhejiang Chinese Medical University, Oncology Department of the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China; Department of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK. Electronic address: georgios.giamas@zcmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plasma small extracellular vesicles (sEVs) are a promising liquid biopsy tool. This study aims to delineate and validate a multimodal plasma sEV biomarker signature for glioma. We use size exclusion chromatography to separate sEVs from plasma (1 mL) and a combination of multi-spectral (Fourier transform infrared/Raman) and orthogonal multi-omics (proteomic/microRNA) approaches on 206 plasma samples (159 individuals) across three independent cohorts. We identify distinct glioma sEV biomolecular profiles, including differences in sEV protein/nucleic acid composition, and consistent alterations in 45 proteins and 20 microRNAs. Machine learning models derived from training cohort data achieve high diagnostic performance (areas under the curve [AUCs] 0.931-0.971), while external validation across independent cohorts confirms the signature's diagnostic potential, with 100% accuracy for the proteomic and multimodal signatures in the longitudinal cohort. Our findings, generated through a rigorous multi-cohort and multi-algorithmic framework, establish the potential of plasma sEV signatures as a clinically relevant diagnostic liquid biopsy approach for glioma.

Indexed as

Biomarkers, TumorBrain NeoplasmsExtracellular VesiclesGliomaAdultFemaleHumansLiquid BiopsyMachine LearningMaleMicroRNAsMiddle AgedMultiomicsProteomicsBiomarkers, TumorMicroRNAsbiomarkerbloodbrain tumorextracellular vesicleglioblastomagliomaliquid biopsymachine-learningmulti-omicsspectroscopy

Identifiers

PMID41999751
PMCPMC13198237

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.