Evidence map›Paper›PMID 41999986›Full record

ArticleSLAS technology2026

Self-organized anteroposterior regionalization of early midbrain and hindbrain/spinal cords using micropatterned human pluripotent stem cells.

Tianfa Xie, Han Jiang, Lauren E Brown, ChangHui Pak, Yubing Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in SLAS technology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tianfa XieDepartment of Mechanical and Industrial Engineering, University of Massachusetts, Amherst, MA, 01003, USA.
Han JiangDepartment of Mechanical and Industrial Engineering, University of Massachusetts, Amherst, MA, 01003, USA.
Lauren E BrownDepartment of Biomedical Engineering, University of Massachusetts, Amherst, MA, 01003, USA.
ChangHui PakDepartment of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA, 01003, USA.
Yubing SunDepartment of Mechanical and Industrial Engineering, University of Massachusetts, Amherst, MA, 01003, USA; Department of Biomedical Engineering, University of Massachusetts, Amherst, MA, 01003, USA. Electronic address: ybsun@umass.edu.

Funding

Intrasurgical tissue engineering of autologous grafts using irreversible electroporation for bladder reconstructionR01DK129990 · NIDDK · UNIVERSITY OF MASSACHUSETTS AMHERST · PI SRIMATHVEERAVALLI, GOVINDARAJAN · 2021 to 2024
$1.4M
Patterning human forebrain organoids by engineering controlled biochemical microenvironmentR21MH130843 · NIMH · UNIVERSITY OF MASSACHUSETTS AMHERST · PI SUN, YUBING · 2022 to 2023
$411k
NIDDK NIH HHS R01 DK129990NIMH NIH HHS R21 MH130843
6 · The paper itself

Abstract

Anterior-posterior (AP) spatial regionalization is crucial for central nervous system development. Previous studies suggest that morphogen gradients can induce AP patterning in animal and organoid models. While self-organization in early embryogenesis and neurogenesis has been found using geometrically confined microtissues, spontaneously induced AP patterning has not been reported. Here, we show that circularly micropatterned human pluripotent stem cells self-organize into spatially distinct FOXG1-FOXA1+OTX2+ midbrain-like and HOXB4+ hindbrain/spinal cord-like regions. Notably, the tissue then folds inwardly to form a 3D annular structure, maintaining a distinct boundary between OTX2+ and HOXB4+ zones. The reaction-diffusion of BMP/Noggin plays a key role in the mechanism of AP patterning. Our model is validated by its capability to distinguish the teratogenic effects of valproic acid and isotretinoin. Our work suggests a novel regulatory mechanism for AP patterning and provides a tool for fast screening of teratogens.

Indexed as

Body PatterningMesencephalonPluripotent Stem CellsRhombencephalonSpinal CordHumansAnterior-posterior patterningHindbrainMicropatterningMidbrainPluripotent stem cellsSpinal cords

Identifiers

PMID41999986
PMCPMC13170484

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.