Evidence map›Paper›PMID 42001237›Full record

ArticleBiophysical journal2026

Capturing transient states of heterodimeric ABC transporter TM287/288 by time-resolved small-angle X-ray scattering.

Lea Schröder, Dario De Vecchis, Andrey Gruzinov, Lars V Schäfer, Clement E Blanchet, Markus A Seeger, Henning Tidow, Inokentijs Josts

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Article in Biophysical journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

8 authors.

Lea SchröderThe Hamburg Advanced Research Centre for Bioorganic Chemistry (HARBOR), Luruper Chaussee 149, 22761 Hamburg, Germany; Department of Chemistry, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
Dario De VecchisCenter for Theoretical Chemistry, Ruhr University Bochum, Universitätsstr. 150, 44801 Bochum, Germany.
Andrey GruzinovEuropean Molecular Biology Laboratory Hamburg Outstation c/o DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Lars V SchäferCenter for Theoretical Chemistry, Ruhr University Bochum, Universitätsstr. 150, 44801 Bochum, Germany.
Clement E BlanchetEuropean Molecular Biology Laboratory Hamburg Outstation c/o DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Markus A SeegerInstitute of Medical Microbiology, University of Zurich, Zürich, Switzerland.
Henning TidowThe Hamburg Advanced Research Centre for Bioorganic Chemistry (HARBOR), Luruper Chaussee 149, 22761 Hamburg, Germany; Department of Chemistry, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany. Electronic address: henning.tidow@uni-hamburg.de.
Inokentijs JostsThe Hamburg Advanced Research Centre for Bioorganic Chemistry (HARBOR), Luruper Chaussee 149, 22761 Hamburg, Germany; Department of Chemistry, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany; Newcastle University, Faculty of Medical Sciences, Newcastle University Biosciences Institute (NUBI), Framlington Place, Newcastle upon Tyne NE2 4HH, UK. Electronic address: kesha.josts@newcastle.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Structures of the heterodimeric ATP-binding cassette (ABC) transporter TM287/288 have previously been determined in several states indicating large conformational changes during its reaction cycle. However, for a complete description of the cycle, transient states (such as an occluded state) are still missing, as they are difficult to capture with static structural biology techniques. Here, we investigate the kinetics and accompanying structural changes in the course of the reaction cycle of full-length TM287/288 using time-resolved small-angle X-ray scattering initiated by stopped-flow mixing. The use of active-site mutants and state-specific sybodies/nanobodies enabled us to dissect the temporal events involved in the ATP-driven conformational cycle of TM287/288 and reveal a transient occluded state of this ABC transporter.

Indexed as

ATP-Binding Cassette TransportersProtein MultimerizationScattering, Small AngleX-Ray DiffractionKineticsModels, MolecularTime FactorsATP-Binding Cassette TransportersABC transporternanobodies/sybodiesSAXStime-resolved small-angle X-ray scattering

Identifiers

PMID42001237
PMCPMC13351893

What Socratic holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.