Evidence map›Paper›PMID 42006855›Full record

ArticleSynthetic and systems biotechnology2026

iFLinkC-EZ: A scalable and automatable method for the assembly of complex fusion proteins and multi-gene expression constructs based on the iFLinkC framework.

Philipp Kemp, Melinda Blat Belmonte, Max Schmidt, Klara Eisenhauer, Carolin Gebhardt, Kai Kabuth, Heinz Koeppl, Viktor Stein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Synthetic and systems biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Philipp KempDepartment of Biology, TU Darmstadt, 64287, Darmstadt, Germany.
Melinda Blat BelmonteDepartment of Biology, TU Darmstadt, 64287, Darmstadt, Germany.
Max SchmidtDepartment of Biology, TU Darmstadt, 64287, Darmstadt, Germany.
Klara EisenhauerDepartment of Biology, TU Darmstadt, 64287, Darmstadt, Germany.
Carolin GebhardtDepartment of Biology, TU Darmstadt, 64287, Darmstadt, Germany.
Kai KabuthDepartment of Biology, TU Darmstadt, 64287, Darmstadt, Germany.
Heinz KoepplCentre for Synthetic Biology, TU Darmstadt, 64283, Darmstadt, Germany.
Viktor SteinDepartment of Biology, TU Darmstadt, 64287, Darmstadt, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Standardized methods for the assembly of DNA constructs have become indispensable in synthetic biology, biotechnology and basic research. The majority of frameworks-most prominently based on Golden Gate-have been developed and optimized for the assembly of transcriptional units (TUs) and thereof composed multi-gene expression constructs. This is significantly enabled by the modular organization of functional elements underlying any given TU. In contrast, the assembly of protein coding sequences typically necessitates tailored approaches. Addressing this technological gap, iterative functional linker cloning (iFLinkC) was recently developed to provide a standardized framework for assembling protein coding sequences of arbitrary size and complexity from the ground up based on pairwise ligations of any two DNA fragments

Indexed as

DNA assemblyFusion proteinsGolden gateiFLinkCLaboratory automationLinker engineering

Identifiers

PMID42006855
PMCPMC13090719

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.