ArticleIranian journal of otorhinolaryngology2026
Pepsin-induced Differential IL-6 Expression in Laryngeal and Laryngopharyngeal Tissue: Non-invasive Rat Model for Laryngopharyngeal Reflux.
Article in Iranian journal of otorhinolaryngology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Laryngopharyngeal reflux is a common condition caused by exposure of the upper aerodigestive tract to gastroduodenal content, resulting in inflammation to the area. Despite the condition being well documented in the human population, the literature on animal models for laryngopharyngeal reflux is currently limited. The aim of this study is to create a non-invasive model of laryngopharyngeal reflux using rats to investigate changes in IL-6 expression in the laryngeal and laryngopharyngeal regions. Materials and Methods: Male Wistar rats, ranging from ages 10-14 weeks, were exposed to 0.5% bovine pepsin dissolved in artificial gastric juice three times daily for 5-15 days via a modified IV cannula in several volumes (0 mL, 0.1 mL, 0.15 mL, and 0.2 mL) according to the treatment groups. Subsequently, laryngeal and laryngopharyngeal tissue IL-6 concentrations were measured through ELISA on allotted days of sacrifice. Results: Significant differences in laryngeal and laryngopharyngeal tissue IL-6 concentration were measured (p = 0.005 and p = 0.020 respectively). Laryngeal expression of IL-6 was related to the volume administered (p = 0.007) but not to the duration of exposure (p = 0.338), with no significant interaction between duration of exposure and administered volume (p = 0.515). On the other hand, laryngopharyngeal expression of IL-6 was related to the duration of exposure (p< 0.001) but not to the volume administered (p = 0.521) with significant interaction between duration and volume (p < 0.001). Conclusion: The study established the feasibility of using a non-invasive rat model for studying laryngopharyngeal reflux. Additionally, the study demonstrated the differential responses of IL-6 to the addition of the acid-pepsin insult, with dose-dependent IL-6 responding more in laryngeal tissue and time-dependent IL-6 responding in laryngopharyngeal tissue.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.