Evidence map›Paper›PMID 42007150›Full record

ArticleAmerican journal of translational research2026

Upregulated lncRNA XIST drives trophoblast dysfunction through targeting miR-545-5p/POU2F1 axis in recurrent spontaneous abortion.

Yanping Qian, Zhuo Chen, Hongping Niu, Liwei Xing, Yiwen Wang, Lijuan Jiang

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Article in American journal of translational research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Yanping QianThe First Clinical Medical College, Nanjing University of Chinese Medicine Nanjing, Jiangsu, China.
Zhuo ChenDepartment of Geriatrics, The First Affiliated Hospital of Yunnan University of Chinese Medicine Kunming, Yunnan, China.
Hongping NiuDepartment of Gynecology, The First Affiliated Hospital of Yunnan University of Chinese Medicine Kunming, Yunnan, China.
Liwei XingThe First Clinical Medical School, Yunnan University of Chinese Medicine Kunming, Yunnan, China.
Yiwen WangDepartment of Gynecology, The First Affiliated Hospital of Yunnan University of Chinese Medicine Kunming, Yunnan, China.
Lijuan JiangThe First Clinical Medical College, Nanjing University of Chinese Medicine Nanjing, Jiangsu, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the specific role of long non-coding RNA (lncRNA) XIST in recurrent spontaneous abortion (RSA).

methodsPlacental tissue samples from RSA patients and healthy controls were obtained to assess expression of the XIST/miR-545-5p/POU2F1 axis. Functional assays evaluating cell proliferation, apoptosis, and invasion, as well as nuclear factor kappa B (NF-κB) pathway activity, were performed in HTR-8/Svneo trophoblasts following transfection with short hairpin RNA (shRNA), miRNA mimics, or inhibitors. The targeting relationship among these molecules was verified through dual luciferase reporter gene assays. An RSA mouse model was established to study the effects of XIST knockdown on this molecular axis and pregnancy outcomes

resultsIn clinical RSA samples, lncRNA XIST expression was significantly upregulated. Knockdown of XIST in trophoblasts led to enhanced cell proliferation and invasion while reducing cell apoptosis. In the RSA mouse model, suppression of XIST decreased the embryonic resorption rate and improved pregnancy outcomes. Mechanistic studies revealed that XIST acts as a competing endogenous RNA that sponges miR-545-5p, thereby relieving repression of its downstream target POU2F1. Dysregulation of the XIST/miR-545-5p/POU2F1 axis contributed to trophoblast dysfunction and RSA pathogenesis through activation of the pro-inflammatory NF-κB signaling pathway.

conclusionThese findings suggest that lncRNA XIST may serve as a potential prognostic marker for RSA and promote disease progression via the miR-545-5p/POU2F1 regulatory axis.

Indexed as

lncRNA XISTmiR-545-5pPOU2F1recurrent spontaneous abortiontrophoblast

Identifiers

PMID42007150
PMCPMC13090928

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.