Evidence map›Paper›PMID 42008498›Full record

ArticlePloS one2026

Identification of an optimal exogenous gene insertion site (P-M) and establishment of a reverse genetics system for aMPV/A.

Yu Guo, Ting Xue, Bingqing Lu, Jiqing Zheng, Yirong Wang, Xueqiao Xie, Huiting Wang, Furui Cao, Yijun Zhang, Jianhua Wang

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Yu GuoThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.ORCID https://orcid.org/0009-0003-8411-5437
Ting XueThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.
Bingqing LuThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.
Jiqing ZhengThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.
Yirong WangThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.
Xueqiao XieThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.
Huiting WangThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.
Furui CaoThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.
Yijun ZhangThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.
Jianhua WangThe College of Biomedical Medicine, Suzhou Chien-Shiung Institute of Technology, Suzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Avian metapneumovirus subgroup A (aMPV/A) causes widespread infections in commercial poultry worldwide. The development of highly efficient viral vector vaccines is constrained by multiple factors, among which the lack of a mature reverse genetics system and the uncertainty regarding the optimal insertion site for foreign genes represent one of the core technical bottlenecks. Here, we constructed an aMPV/A reverse genetics system based on the pBR322 vector and inserted EGFP into seven of its intergenic regions to generate recombinant viruses. Viral replication, exogenous gene expression and genetic stability were systematically assessed via multiple assays. The results demonstrated that the expression level at the P‑M locus after EGFP insertion was approximately 1.2‑fold higher than that of other recombinant viruses. In addition, there was no significant difference in viral titer, and good genetic stability was observed over 20 generations. This study establishes a stable and efficient reverse genetics system for aMPV/A, which provides a critical technical platform for aMPV/A molecular mechanism research and novel recombinant vector vaccine development, with important practical value for global aMPV prevention and control.

Indexed as

MetapneumovirusMutagenesis, InsertionalReverse GeneticsAnimalsChickensGenetic VectorsGreen Fluorescent ProteinsVirus Replicationenhanced green fluorescent proteinGreen Fluorescent Proteins

Identifiers

PMID42008498
PMCPMC13094954

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.