Evidence map›Paper›PMID 42009678›Full record

ArticleNature communications2026

Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking.

Justin A Bosch, Pierre Michel Jean Beltran, Cooper Cavers, James Thai LaGraff, Randy Melanson, Ankita Singh, Weihang Chen, Yanhui Hu, Sudhir Gopal Tattikota, Ying Liu and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Justin A BoschDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA. jabosch@genetics.utah.edu.ORCID http://orcid.org/0000-0001-8499-1566
Pierre Michel Jean BeltranBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Cooper CaversDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
James Thai LaGraffDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-6148-7671
Randy MelansonBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Ankita SinghDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-6045-1757
Weihang ChenDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Yanhui HuDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0003-1494-1402
Sudhir Gopal TattikotaDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0003-0318-5533
Ying LiuDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-6419-1980
Yousuf HashmiDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0001-5163-8854
John M AsaraDivision of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA, USA.ORCID http://orcid.org/0000-0001-7450-2589
Tess BranonDepartments of Genetics, Biology, and by courtesy, Chemistry, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-6520-6419
Alice Y TingDepartments of Genetics, Biology, and by courtesy, Chemistry, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-8277-5226
Steven A CarrBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-7203-4299
Norbert PerrimonDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA. perrimon@genetics.med.harvard.edu.ORCID http://orcid.org/0000-0001-7542-472X

Funding

New collections of dsRNAs and overexpression miRNA constructs to supplement RNAiR01GM067761 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI PERRIMON, NORBERT · 2003 to 2019
$15.5M
TRAINING GRANT IN GENETICST32GM007748 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI Anne O'Donnell-Luria, Louise Wilkins-Haug · 1985 to 2026
$12.0M
Drosophila Transgenic RNAi Resource ProjectR01GM084947 · NIGMS · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2008 to 2019
$9.8M
Training Program in Cellular and Molecular BiologyT32GM139774 · NIGMS · WASHINGTON UNIVERSITY · PI HEATHER L TRUE-KROB · 2021 to 2026
$6.7M
Next-generation Drosophila cell lines to elucidate the cellular basis of human diseasesR24OD019847 · OD · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT, SIMCOX, AMANDA · 2017 to 2020
$2.7M
Damon Runyon Cancer Research Foundation (Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation) DRG-2258-16NIGMS NIH HHS R01 GM067761NIGMS NIH HHS R01 GM084947NIGMS NIH HHS T32 GM007748NIGMS NIH HHS T32 GM139774NIH HHS R24 OD019847
6 · The paper itself

Abstract

Secreted proteins regulate many aspects of animal biology and are attractive targets for biomarkers and therapeutics. However, comprehensively identifying the "secretome", along with their tissues of origin, remains extremely challenging. To address this, we employed multiple 'omics methods to define a tissue-secretome map of 535 blood plasma proteins derived from specific cell-types and organs in Drosophila melanogaster. This map was enabled by methodological improvements including a collection of transgenic flies to label endogenous secreted proteins in 10 major tissue types, large-scale blood isolation, whole animal snRNA-seq, and 40 CRISPR knock-in strains. Using this map, we identify features of circulating proteins: most originate from specific tissues including unusual sources (e.g. glia), many are uncharacterized, and some are shed ectodomains of transmembrane proteins. In addition, in vivo experiments revealed circulating proteins with tissue-specific expression, as well as proteins that are deposited in a different tissue from where they are synthesized, suggesting potential inter-organ functions. Our secretome map will serve as a resource to investigate blood protein function, discover candidate tissue-tissue communication signals, and mine for homologues of human biomarkers.

Indexed as

Blood ProteinsDrosophila melanogasterDrosophila ProteinsProteomeAnimalsAnimals, Genetically ModifiedHumansMultiomicsOrgan SpecificityProtein TransportProteomicsBlood ProteinsDrosophila ProteinsProteome

Identifiers

PMID42009678
PMCPMC13280006

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.