Evidence map›Paper›PMID 42009850›Full record

ArticleScientific reports2026

Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.

Bianca Brown, Elizabeth Nagy, Ajuni K Takkar, Naomi A Greengold, Shreyas S Gujar, Ali Amini, Mary A Collins, Javad Rajabi, Jenna Walls, Chenai Kaminski and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Bianca BrownBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Elizabeth NagyBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Ajuni K TakkarBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Naomi A GreengoldBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Shreyas S GujarBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Ali AminiCenter for Data Science, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.ORCID http://orcid.org/0009-0008-9603-5756
Mary A CollinsBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Javad RajabiDepartment of Computer Science, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Jenna WallsBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Chenai KaminskiBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Michael SanchezBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Elle McQuire-GuzmanBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
Taraji EllingtonBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA.
John R BrachtBiology Department, American University, 4400 Massachusetts Avenue, NW, Washington, DC, 20016, USA. jbracht@american.edu.ORCID http://orcid.org/0000-0001-8042-7685

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Organoids are three-dimensional structures that develop in cultures of stem cells, and resemble multicellular organs. While performing long-term culture of Taeniopygia guttata (zebra finch) germline tissue, we observed the formation of germline organoids from cultures of both sexes. These macro-scale structures contain multiple cell types and retain Primordial Germ Cells (PGCs) and their descendant germ-cell lineages for two to four months of culture in vitro. We show that the PGC-specific germline-restricted chromosome (GRC) can be detected after three months of culture, and that the organoids exhibit DAZL- and DDX4-positive structures resembling germinal epithelia. Taken together our results open new possibilities for the study of key steps of avian reproductive development.

Indexed as

FinchesGerm CellsOrganoidsAnimalsFemaleMale

Identifiers

PMID42009850
PMCPMC13265798

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.