Evidence map›Paper›PMID 42010074›Full record

ArticleDoklady. Biochemistry and biophysics2026

Development of an Approach for Parasegment-Specific Labeling of Nuclei in Drosophila melanogaster Embryos.

I O Deriglazova, O V Kyrchanova, P G Georgiev, O G Maksimenko

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Article in Doklady. Biochemistry and biophysics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

4 authors.

I O DeriglazovaInstitute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
O V KyrchanovaInstitute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
P G GeorgievInstitute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
O G MaksimenkoInstitute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. maksog@mail.ru.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Effective approaches for selecting nuclei in which a gene of interest is in an active state are necessary for studying spatial organization and gene expression regulation. In this work, a novel two-component genetic system was created for the parasegment-specific labeling of nuclei in which the regulatory domain iab-5, which stimulates the homeotic gene Abd-B, is active. The system is based on the integration of a transgene expressing the yeast GAL4 activator under the control of the minimal hsp70 gene promoter into the iab-5 domain, near the early embryonic enhancer. As a result, the iab-5 enhancer induces specific expression of GAL4, which strongly amplifies the expression of the mScarlet fluorescent protein, allowing for the efficient selection of labeled nuclei in which the iab-5 domain is activated. This approach can be used for the selection of target nuclei in which any regulatory element or gene of interest is in an active or repressed state.

Indexed as

Cell NucleusDrosophila melanogasterEmbryo, NonmammalianAnimalsDrosophila ProteinsEnhancer Elements, GeneticGene Expression Regulation, DevelopmentalPromoter Regions, GeneticTranscription FactorsDrosophila ProteinsTranscription FactorsAbd-B geneBithorax complexenhanceriab-5 domainregulatory elementtranscription factor

Identifiers

PMID42010074

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.