Evidence map›Paper›PMID 42012696›Full record

ArticleJournal of bioenergetics and biomembranes2026

SPC25 promotes gastric cancer cells G1/S cell cycle transition and tumorigenesis by enhancing SLC1A5-mediated glutamine metabolism.

Xinqi Yao, Jie Wu, Yu Song, Hao Shen

Erratum issuedAbstract read
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In one paragraph

Article in Journal of bioenergetics and biomembranes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Xinqi YaoDepartment of Gastroenterology, Xi'an Central Hospital, No.161, West 5th Road, Xincheng District, Xi'an, Shaanxi, 710004, China.
Jie WuDepartment of Pathology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China.
Yu SongDepartment of Gastroenterology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, 710003, China.
Hao ShenDepartment of Gastroenterology, Xi'an Central Hospital, No.161, West 5th Road, Xincheng District, Xi'an, Shaanxi, 710004, China. betterhaos@126.com.

Funding

Natural Science Foundation of Shaanxi Province 2025JC-YBMS-966
6 · The paper itself

Abstract

Although spindle pole body component 25 (SPC25) has been implicated in various cancers, its precise role in GC remains unexplored. This study aimed to investigate the role and mechanism of SPC25 in GC progression. SPC25 expression was analyzed using TCGA database and 40 paired GC clinical specimens. RT-qPCR, Western blot and immunohistochemistry (IHC) staining were performed to detect the expression of SPC25 in GC samples. Flow cytometry was conducted to assess the cell cycle, and commercial kits were used to measure the levels of glutamine, glutamic acid, α-Ketoglutaric acid (α-KG) and NADPH/NADP+. MeRIP-PCR was employed to explore the regulatory role of SPC25 on the m6A levels of SLC1A5. RNA immunoprecipitation (RIP) assay to test the binding between IGF2BP2 and SPC25 transcripts. Xenograft mouse model was constructed to assess the effect of SPC25 on tumor growth in vivo. SPC25 was significantly overexpressed in GC tissues and cell lines. Silencing SPC25 repressed G1/S cell cycle transition and downregulated the expressions of several key mitotic regulators. Moreover, the interference of SPC25 repressed glutamine metabolism in GC cells, as demonstrated by reduced glutamine consumption and decreased production of α-KG, glutamate and NADPH/NADP+. SPC25 overexpression exerted the opposite effects. Mechanistically, SPC25 promoted glutamine metabolism and cell cycle progression in GC by stabilizing SLC1A5 expression through IGF2BP2-mediated m6A modification. Animal study revealed that SPC25 knockdown suppressed tumor growth in vivo. SPC25, stabilizing SLC1A5 expression through IGF2BP2 mediated m6A modification, regulated glutamine metabolism, promoted gastric cancer cells G1/S cell cycle transition and drove tumor progression.

Indexed as

Amino Acid Transport System ASCCarcinogenesisGlutamineMinor Histocompatibility AntigensStomach NeoplasmsAnimalsCell Line, TumorFemaleHumansMiceMice, NudeAmino Acid Transport System ASCGlutamineMinor Histocompatibility AntigensSLC1A5 protein, humangastric cancerglutamine metabolismIGF2BP2m6ASLC1A5SPC25

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.