Evidence map›Paper›PMID 42017759›Full record

ArticleJournal of diabetes research2026

Wound Healing Effects of Polyethylene Glycol Loxenatide via PI3K/AKT Pathway: In Vitro Evaluation in Human Keratinocytes.

Zhiyi Zhao, Han Yue, Weijie Xu, Song Gong, Shiying Shao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zhiyi ZhaoDivision of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, hust.edu.cn.ORCID https://orcid.org/0009-0003-4395-6758
Han YueDivision of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, hust.edu.cn.ORCID https://orcid.org/0009-0008-3646-652X
Weijie XuDivision of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, hust.edu.cn.ORCID https://orcid.org/0000-0001-8722-2084
Song GongDivision of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, hust.edu.cn.ORCID https://orcid.org/0000-0003-1881-4910
Shiying ShaoDivision of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, hust.edu.cn.ORCID https://orcid.org/0000-0001-7077-5184

Funding

Bethune Charitable Foundation 2024China International Medical Foundation-Senmei China Diabetes Research Fund Z-2017-26-1902-5Hubei Provincial Natural Science Foundation and YICHANG-of China 2025AFD271Sichuan Provincial Western Psychiatric Association's CSPC LEADING Scientific Research Project WL2021104
6 · The paper itself

Abstract

aimGlucagon-like peptide-1 receptor agonists (GLP-1RAs) hold clinical promise in promoting wound healing. This study investigated the pro-healing effect and potential molecular mechanism of polyethylene glycol loxenatide (PEG-Lox). MATERIALS AND

methodsHaCaT cells were incubated in high glucose (HG) condition with or without PEG-Lox. Cell proliferation and viability were assessed by Cell Counting Kit 8 (CCK8) assay and PI staining. Cell migration was evaluated via Transwell and scratch wound healing assays. Transcriptomic analysis was conducted to identify the potential differentially expressed genes (DEGs). Western blotting was performed to validate protein expression levels of identified DEGs.

resultsCell proliferation and viability were significantly improved in the PEG-Lox-treated group when compared with the control group. Transwell assay showed that HG inhibited the migration of HaCaT cells, whereas PEG-Lox treatment markedly resulted in a 1.7-fold increase in the number of migrating cells (p < 0.0001). The scratch closure rate was 32.83% after PEG-Lox intervention, which was higher than that in the control group (24.42%, p < 0.001). Transcriptomic analysis found early growth response factor 1 (EGR1) to be the key DEG under PEG-Lox treatment. The inhibition of PI3K/AKT signaling pathway by LY294002 down-regulated the expression of EGR1 and impaired the proliferation and migration of HaCaT cells.

conclusionsOur study suggested that GLP-1RA PEG-Lox could promote wound healing by activating the PI3K/AKT/EGR1 signaling pathway, which further uncovered the potential pro-healing effect of GLP-1RAs in wounds.

Indexed as

KeratinocytesPeptidesPhosphatidylinositol 3-KinasesPolyethylene GlycolsProto-Oncogene Proteins c-aktWound HealingCell LineCell MovementCell ProliferationCell SurvivalEarly Growth Response Protein 1Glucagon-Like Peptide-1 Receptor AgonistsGlucoseHaCaT CellsHumansSignal TransductionEarly Growth Response Protein 1Glucagon-Like Peptide-1 Receptor AgonistsGlucosePeptidesPhosphatidylinositol 3-KinasesPolyethylene GlycolsProto-Oncogene Proteins c-aktglucagon-like peptide-1keratinocyteloxenatidewound healing

Identifiers

PMID42017759
PMCPMC13101450

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.