Evidence map›Paper›PMID 42018616›Full record

ArticleScience advances2026

Piezo1-mediated mechanohydraulic control of cell volume drives cardiac morphogenesis.

Christina Vagena-Pantoula, Konstantinos Kalyviotis, Shuyi Feng, Antoine Sanchez, Igor Kondrychyn, Moe Fukumoto, Xiangbin Pan, Thomas Juan, Didier Y R Stainier, Periklis Pantazis and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Christina Vagena-PantoulaDepartment of Bioengineering, Imperial College London, London, UK.ORCID 0000-0002-3591-2267
Konstantinos KalyviotisDepartment of Bioengineering, Imperial College London, London, UK.ORCID 0000-0002-7640-8905
Shuyi FengDepartment of Bioengineering, Imperial College London, London, UK.
Antoine SanchezDepartment of Bioengineering, Imperial College London, London, UK.ORCID 0009-0000-7474-0800
Igor KondrychynLaboratory for Vascular Morphogenesis, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.ORCID 0000-0002-2268-7291
Moe FukumotoDepartment of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan.ORCID 0000-0001-8081-1353
Xiangbin PanDepartment of Structural Heart Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.ORCID 0000-0002-7090-4414
Thomas JuanDepartment of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.ORCID 0000-0002-9654-3717
Didier Y R StainierDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.ORCID 0000-0002-0382-0026
Periklis PantazisDepartment of Bioengineering, Imperial College London, London, UK.ORCID 0000-0002-8367-9332
Li-Kun PhngLaboratory for Vascular Morphogenesis, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.ORCID 0000-0001-8523-9958
Julien VermotDepartment of Bioengineering, Imperial College London, London, UK.ORCID 0000-0002-8924-732X

Funding

Wellcome Trust
6 · The paper itself

Abstract

Organ morphogenesis is driven by physical forces, yet how mechanical stimuli pattern tissue shape and guide developmental programs remains poorly understood. In zebrafish, endocardial cells (EdCs) within the heart valve-forming region undergo marked volume reduction during early morphogenesis. Here, we uncover a hydraulics-based mechanism by which mechanical forces control EdC volume to direct cardiac development. We show that the mechanosensitive ion channel Piezo1 acts with the calcium-binding protein calmodulin (CaM) and the aquaporin Aqp8a.1 water channel to orchestrate EdC shrinkage. We find that Aqp8a.1 mediates cell volume loss by incorporating into the plasma membrane in response to mechanical stimulation, promoting heart looping and valve formation. Mechanistically, Piezo1 governs Aqp8a.1 through a dual mechanism. First, Piezo1 and CaM drive Aqp8a.1 plasma membrane incorporation, enabling rapid cell volume adjustments. Second, Piezo1 suppresses

Indexed as

Cell SizeHeartIon ChannelsMechanotransduction, CellularMorphogenesisZebrafish ProteinsAnimalsAquaporinsCalmodulinCell MembraneGene Expression Regulation, DevelopmentalZebrafishAquaporinsCalmodulinIon ChannelsPiezo1 protein, zebrafishZebrafish Proteins

Identifiers

PMID42018616
PMCPMC13101866

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.