Evidence map›Paper›PMID 42019474›Full record

ArticlePoultry science2026

Establishment of a broiler jejunal organoid model based on optimized activators or optimized two-step media and its assessment.

Weizhen Song, Weiyun Zhang, Yun Hu, Tingting Li, Xi Lin, Hsiao-Ching Liu, Jack Odle, Miles Todd See, Shengchen Wang, Xiaoyan Cui and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Weizhen SongPoultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225000, China.
Weiyun ZhangPoultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225000, China.
Yun HuPoultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225000, China.
Tingting LiPoultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225000, China.
Xi LinDepartment of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
Hsiao-Ching LiuDepartment of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
Jack OdleDepartment of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
Miles Todd SeeDepartment of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
Shengchen WangPoultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225000, China.
Xiaoyan CuiPoultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225000, China.
Liyang ZhangMineral Nutrition Research Division, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Xugang LuoPoultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225000, China. Electronic address: wlysz@263.net.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intestinal organoids (IOs), as a novel three-dimensional (3D) in vitro model, have been widely applied for studying intestinal physiology, metabolism and health in human, rodents and farm animals. However, the culture methods for poultry, especially for broiler IOs, need to be improved due to their low differentiation to form complex, self-organized 3D structures and intact barrier functions. This study aimed to establish the broiler jejunal organoid (JO) model by optimizing the culture conditions of JOs and evaluating the cellular components and barrier integrity of JOs via a two-step media [TSM, including both JO growth medium and JO differentiation medium (JODM)]. The results showed that JODM containing 50 or 100 ng/mL of R-spondin 1 and 2.5 μM of Chiron 99021 increased (P < 0.001) the budding percentage of the JOs up to about 80% and maintained their passaging and cryopreservation capabilities. Furthermore, a variety of intestinal functional cells differentiated into JOs as evidenced by the expressions of different marker proteins, and JOs displayed evidence of barrier integrity. Compared with conditioned medium-dependent medium (CMDM) and IntestiCult™ Organoid Growth Medium (IOGM), TSM increased (P < 0.001) the budding percentage of JOs up to about 80%. Moreover, IOGM and TSM up-regulated (P < 0.001) marker genes expression of different intestinal functional cells compared with CMDM, and jejunal crypts sourced from broilers of different ages had no effect (P = 0.792) on the differentiation and budding percentage of JOs. In conclusion, the broiler JOs model established using the TSM-culture protocol developed in this study has the advantages of high differentiation, enriched budding structures and stable passaging and cryopreservation, providing a superior experimental model for studying the physiological responses of the broiler intestine to nutrients, feed additives, pathogens, drugs and their mechanisms.

Indexed as

ChickensCulture MediaJejunumOrganoidsAnimalsCulture MediaBarrier integrityBroilerBudding percentageJejunal organoidTwo-step media

Identifiers

PMID42019474
PMCPMC13125189

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.