Evidence mapPaperPMID 42019489Full record

ArticleDevelopmental cell2026

A skeletal muscle atlas shows neuromuscular junction adaptations to growth and atrophy.

Silvia Campanario, Mercedes Grima-Terrén, Megan Rommelfanger, Stefania Dell' Orso, Xuesong Feng, Andrés Cisneros, Ignacio Ramírez-Pardo, Aina Calls-Cobos, Benjamin A Yang, Kyung Dae Ko and 17 more

Abstract read
In one paragraph

Article in Developmental cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Silvia CampanarioAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid 28029, Spain.
Mercedes Grima-TerrénAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid 28029, Spain.
Megan RommelfangerAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA.
Stefania Dell' OrsoGenomic Technology Section, NIAMS, NIH, Bethesda, MD 20892, USA.
Xuesong FengLaboratory of Muscle Stem Cells & Gene Regulation, NIAMS, NIH, Bethesda, MD 20892, USA.
Andrés CisnerosAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain.
Ignacio Ramírez-PardoAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid 28029, Spain.
Aina Calls-CobosAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain.
Benjamin A YangAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA.
Kyung Dae KoLaboratory of Muscle Stem Cells & Gene Regulation, NIAMS, NIH, Bethesda, MD 20892, USA.
Esther García-DomínguezFreshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, Fundación Investigación Hospital Clínico Universitario/INCLIVA, Valencia 46010, Spain; CIBER de Fragilidad y Envejecimiento Saludable (CIBERFES) ISCIII, Madrid 28029, Spain.
Laura Pena-CousoCentro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid 28029, Spain; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Grace ChouAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA.
Yuewen ZhengAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA.
Nasun HahAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA.
Davide RandazzoLight Imaging Section, NIAMS, NIH, Bethesda, MD 20892, USA.
Alberto Pérez-GarciaDepartment of Plastic Surgery and Burns, University and Polytechnic Hospital La Fe, Valencia 46026, Spain.
Tovah E MarkowitzIntegrated Data Sciences Section, Research Technologies Branch, NIAID, NIH, Bethesda, MD 20892, USA.
Jose MilisendaMuscle Research Unit, Internal Medicine Service, Hospital Clinic, Barcelona 08036, Spain; Barcelona University, CIBERER and IDIBAPS, Barcelona 08036, Spain.
Iago Pinal-FernandezMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Andrew MammenMuscle Disease Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Mari Carmen Gómez-CabreraFreshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, Fundación Investigación Hospital Clínico Universitario/INCLIVA, Valencia 46010, Spain; CIBER de Fragilidad y Envejecimiento Saludable (CIBERFES) ISCIII, Madrid 28029, Spain.
Antonio L SerranoAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain.
Eusebio PerdigueroAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain.
Vittorio SartorelliLaboratory of Muscle Stem Cells & Gene Regulation, NIAMS, NIH, Bethesda, MD 20892, USA. Electronic address: sartorev@mail.nih.gov.
Joan IsernAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid 28029, Spain. Electronic address: jisern-external@altoslabs.com.
Pura Muñoz-CánovesAltos Labs, San Diego Institute of Science, San Diego, CA 92121, USA; Department of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF) Barcelona 08003, Spain; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid 28029, Spain; ICREA, Barcelona 08908, Spain. Electronic address: pmunozcanoves@altoslabs.com.

Funding

Control of Myogenesis and Regulation of MyoD Post-Transcriptional ModificationsZIAAR041126 · NIAMS · NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES · PI SARTORELLI, VITTORIO · 2009 to 2025
$28.7M
SIRT1 in Skeletal Muscle Development, Regeneration, and AtrophyZIAAR041164 · NIAMS · NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES · PI SARTORELLI, VITTORIO · 2009 to 2025
$15.3M
Intramural NIH HHS ZIA AR041126Intramural NIH HHS ZIA AR041164
6 · The paper itself

Abstract

The molecular basis underlying muscle atrophy, as it occurs during disuse or aging, and activity-induced hypertrophy remain poorly understood. A major challenge has been defining the diverse cellular and niche environments within skeletal muscle, which is mostly composed of multinucleated myofibers. Here, we present a single-nucleus and single-cell transcriptomic atlas, coupled with spatial profiling, of mouse limb skeletal muscle under resting conditions and during experimentally induced atrophy or hypertrophy. We identify condition-dependent shifts in muscle-resident cell populations and fiber-type-specific transcriptional responses. We also uncover extensive remodeling of the neuromuscular junction (NMJ), including the emergence of specialized synaptic myonuclei (SynM) and terminal Schwann cells (tSCs) associated with atrophic or hypertrophic states. High-resolution 3D imaging and spatial transcriptomics confirm these changes at the tissue level. Similar NMJ alterations are observed in denervated and exercised human muscle, supporting the translational relevance of this atlas for studying muscle plasticity and identifying therapeutic targets in muscle-related diseases.

Indexed as

Adaptation, PhysiologicalMuscle, SkeletalMuscular AtrophyNeuromuscular JunctionAnimalsHumansHypertrophyMiceSchwann CellsTranscriptomeatrophycell atlashypertrophyneuromuscular junctionSchwann cellskeletal muscle

Identifiers

PMID42019489
PMCPMC13108521

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.