Evidence map›Paper›PMID 42022757›Full record

ArticleExperimental and therapeutic medicine2026

Interacting proteins of AMPK studied using TurboID proximity labeling technology.

Xiaoshan Liu, Jieyu Guo, Qiongyao Wang, Wu Liu, Yan Xue, Shuang Guo, Miao Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental and therapeutic medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiaoshan LiuSchool of Pharmacy, Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, Hubei 437100, P.R. China.
Jieyu GuoSchool of Pharmacy, Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, Hubei 437100, P.R. China.
Qiongyao WangDepartment of Oncology, Xianning Central Hospital of Hubei Province (The First Affiliated Hospital of Hubei University of Science and Technology), Xianning, Hubei 437100, P.R. China.
Wu LiuSchool of Basic Medical Sciences, Hubei Key Laboratory of Environmental Risks and Related Diseases Precision Control, Xianning Medical College, Hubei University of Science and Technology, Xianning, Hubei 437100, P.R. China.
Yan XueSchool of Pharmacy, Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, Hubei 437100, P.R. China.
Shuang GuoSchool of Pharmacy, Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, Hubei 437100, P.R. China.
Miao LiDepartment of Nursing, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570102, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In living organisms, the change in the adenosine 5'-monophosphate (AMP)/adenosine 5'-triphosphate ratio serves as a key signal regulating AMP-activated protein kinase (AMPK) activity, which is an important molecule for the control of cellular energy metabolism and serves a key role in a number of diseases, such as diabetes and myocardial infarction. To explore novel AMPK-interacting proteins and investigate their biological functions, the present study used TurboID proximity labeling in U251 cells stably expressing AMPK-TurboID. Novel AMPK-interacting proteins were explored under both normal and CCCP-treated conditions to investigate their biological functions. Stable cell lines overexpressing AMPK-TurboID were successfully established, and interacting proteins were identified through biotin labeling, silver staining and mass spectrometry analysis. Due to the involvement of AMPK in numerous metabolic pathways and the advantages of TurboID over traditional techniques, including its high temporal resolution,

Indexed as

AMPKbiotin labelingDNAJ heat shock protein family (Hsp40) member A1TurboIDU251 cells

Identifiers

PMID42022757
PMCPMC13096766

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.