Evidence map›Paper›PMID 42032768›Full record

ArticleJournal of biological engineering2026

A continuous viral vaccine biomanufacturing platform utilizing multiple bioreactor configurations.

Justin Sargunas, Bradley Priem, Dylan Carman, Taravat Sarvari, Natalie M Nold, Vaishali Sharma, Andrew Pekosz, Caryn L Heldt, Michael Betenbaugh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Justin Sargunas *Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N Charles St, Baltimore, Maryland, 21218, USA.ORCID http://orcid.org/0009-0002-6734-3510
Bradley Priem *Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N Charles St, Baltimore, Maryland, 21218, USA.ORCID http://orcid.org/0009-0002-2849-6119
Dylan CarmanDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N Charles St, Baltimore, Maryland, 21218, USA.
Taravat SarvariDepartment of Chemical Engineering, Michigan Technological University, 1400 Townsend Dr, Houghton, Michigan, 49931, USA.
Natalie M NoldDepartment of Chemical Engineering, Michigan Technological University, 1400 Townsend Dr, Houghton, Michigan, 49931, USA.ORCID http://orcid.org/0000-0003-0007-2285
Vaishali SharmaDepartment of Biological Sciences, Michigan Technological University, 1400 Townsend Dr, Houghton, Michigan, 49931, USA.ORCID http://orcid.org/0000-0003-4069-314X
Andrew PekoszW. Harry Feinstone Department of Molecular Microbiology and Immunology, the Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe St, Baltimore, Maryland, 21205, USA.ORCID http://orcid.org/0000-0003-3248-1761
Caryn L HeldtDepartment of Chemical Engineering, Michigan Technological University, 1400 Townsend Dr, Houghton, Michigan, 49931, USA.ORCID http://orcid.org/0000-0002-0776-8763
Michael BetenbaughDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N Charles St, Baltimore, Maryland, 21218, USA. beten@jhu.edu.ORCID http://orcid.org/0000-0002-6336-4659

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Scalable, continuous biomanufacturing processes have grown in importance to meet demand for smaller bioreactor sizes, lowered production costs, and improved quality attribute consistency. The Sf9/recombinant baculovirus (rBV) expression system demonstrates promise for virus-like particle (VLP) vaccine and gene therapy production. Here, we present a continuous rBV platform integrating an infection plug flow reactor (PFR) between stirred tank growth (gCSTR) and production (pCSTR) bioreactors. Cell expansion in the gCSTR included a ramp-up stage followed by continuous growth, reaching a steady state of 5×10

Indexed as

Continuous biomanufacturingDigital twinPlug flow reactor (PFR)Sf9 insect cellsViral vaccinesVirus-like particle (VLP)

Identifiers

PMID42032768
PMCPMC13244601

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.