Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells.

Christina Pfab, Jonas M Holzinger, Sarah Hirsch, Katharina U Roithmeier, André Gessner, Sigrid Bülow

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Christina PfabInstitute of Medical Microbiology and Hygiene Regensburg, University of Regensburg, Regensburg 93053, Germany.

Jonas M HolzingerInstitute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg 93053, Germany.

Sarah HirschInstitute of Medical Microbiology and Hygiene Regensburg, University of Regensburg, Regensburg 93053, Germany.

Katharina U RoithmeierInstitute of Medical Microbiology and Hygiene Regensburg, University of Regensburg, Regensburg 93053, Germany.

André GessnerInstitute of Medical Microbiology and Hygiene Regensburg, University of Regensburg, Regensburg 93053, Germany; Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg 93053, Germany.

Sigrid BülowInstitute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg 93053, Germany. Electronic address: sigrid.buelow@ukr.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In vitro T cell activation and differentiation are fundamental techniques in immunological research. Here, we present a protocol for the potent activation of T cells using bactericidal/permeability-increasing protein (BPI)-stimulated murine bone marrow-derived cells (BMDCs). We describe steps for preparing supernatants harvested from BPI-stimulated BMDCs (SN BPI). We then detail procedures for purification of (naive) CD4

Indexed as

Antimicrobial Cationic PeptidesBone Marrow CellsCell Culture TechniquesLymphocyte ActivationT-LymphocytesAnimalsCD4-Positive T-LymphocytesMiceAntimicrobial Cationic PeptidesCell-based AssaysCell cultureCell isolationImmunologyModel Organisms

Identifiers

PMID42033728

DOI10.1016/j.xpro.2026.104519

PMCPMC13129426

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.