Evidence map›Paper›PMID 42033730›Full record

ArticleSTAR protocols2026

Protocol for Seahorse 3D Mito Stress assay in patient-derived atypical teratoid rhabdoid tumor CHLA-05-ATRT single neurospheres.

Stefania Tocci, Jessica W Tsai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Stefania TocciChildren's Hospital Los Angeles, Cancer and Blood Disease Institute, Los Angeles, CA, USA; Children's Hospital Los Angeles, Saban Research Institute, Los Angeles, CA, USA.
Jessica W TsaiChildren's Hospital Los Angeles, Cancer and Blood Disease Institute, Los Angeles, CA, USA; Children's Hospital Los Angeles, Saban Research Institute, Los Angeles, CA, USA; Keck School of Medicine of University of Southern California, Department of Pediatrics, Los Angeles, CA, USA; USC Norris Comprehensive Cancer Center, Epigenetic Regulation in Cancer Program, Los Angeles, CA, USA. Electronic address: jtsai@chla.usc.edu.

Funding

Dissecting the cooperation of FOXR2 and ETS transcription factors in FOXR2-expressing diffuse midline gliomasK08CA279908 · NCI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI Jessica Whei Tsai · 2024 to 2026
$789k
NCI NIH HHS K08 CA279908
6 · The paper itself

Abstract

While Seahorse assays are well established for measuring metabolic changes in 2D cell cultures, their application to 3D models remains challenging. Here, we present a protocol for plating individual brain tumor neurospheres in the assay microplate to measure their bioenergetics. We describe steps for neurosphere culturing, cartridge hydration, microplate coating, and neurosphere transfer. We then detail procedures for incubation and Seahorse assay, followed by normalization and analysis. This approach enables exploration of dynamic metabolic changes in patient-derived brain tumor neurospheres at basal conditions or following genetic or pharmacologic interventions.

Indexed as

Brain NeoplasmsCell Culture TechniquesRhabdoid TumorTeratomaCell Line, TumorHumansSpheroids, CellularTumor Cells, CulturedCell-based AssaysCell cultureMetabolism

Identifiers

PMID42033730
PMCPMC13127629

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.